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Behavioral Analysis of Folsomia Candida (Collembola) with Herbicide Using Electronic and Computational Instrumentation: Bioassays
Soil & Sediment Contamination ( IF 2 ) Pub Date : 2020-04-20 , DOI: 10.1080/15320383.2020.1748568
Rodrigo Luiz Ximenes 1 , Adelle Anik Araújo Gomes 2 , Talía Simões Dos Santos Ximenes 1 , Marta Siviero Guilherme Pires 2
Affiliation  

In view of the constant biological researches, it is important to highlight the necessity to develop new instrumentation techniques. In this way, it is possible to use organisms as biological sensors to define new methodologies for a better understanding of the behavior of living beings. Indiscriminate contamination of soil by herbicide has been receiving more attention due to the increase in agriculture and the world population growth. This work presents a technique to analyze the behavior of the Folsomia candida when it is exposed to an area with an herbicide containing glyphosate. The tests used concentrations of the contaminant at the 1:50 and 1:100 dilutions and the media preparation followed culture standards. The computational system developed proved to be efficient and promising to detect Folsomia candida movements when subjected to environmental contamination. In the analysis of the results, we sought to understand the organism’s preference between contaminated and uncontaminated area, and the preference of the resting places and media. The results indicated preference of the organism for the uncontaminated areas, in the two dilutions used. Thus, the system created and the applied methodology can be used in preliminary toxicity tests, for example, tests on chemical compounds and their possible effects in the soil.



中文翻译:

电子和计算仪器对念珠菌与除草剂的行为分析:生物测定

鉴于不断的生物学研究,重要的是强调开发新仪器技术的必要性。这样,可以将生物用作生物传感器来定义新的方法,以更好地了解生物的行为。由于农业的增长和世界人口的增长,除草剂对土壤的不加选择的污染已经引起了更多关注。这项工作提出了一种技术,可以在白色念珠菌暴露于含有草甘膦的除草剂区域时分析其行为。测试使用的污染物浓度为1:50和1:100稀释,培养基制备遵循培养标准。所开发的计算系统被证明是有效的并且有望用于检测遭受环境污染的念珠菌运动。在对结果进行分析时,我们试图了解有机体在受污染区域和未受污染区域之间的偏好,以及休息场所和媒体的偏好。结果表明,在所用的两种稀释液中,生物体更喜欢未污染的区域。因此,创建的系统和应用的方法可以用于初步毒性测试,例如,对化合物及其在土壤中可能产生的影响的测试。

更新日期:2020-04-20
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