1. Birds’ newly oviposited blastoderms can survive several weeks in a dormant state during low-temperature storage. Previous studies demonstrated that there is a critical temperature range from 19 to 27°C for chicken embryos. Within this range, the embryo will diapause in a dormant state; once the temperature rises above this range, the blastoderm will break dormancy.

2. Clarifying the mechanism that initiates duck embryo developmental recovery from blastoderm dormancy will be helpful to change temperature control to improve hatching in poultry production. It was hypothesised that there might be some temperature-sensitive genes involved in initiating duck embryo developmental recovery from blastoderm dormancy.

3. To test this hypothesis, the transcriptome of the newly oviposited duck blastoderm and duck embryo (incubated for 48 hours) were sequenced to screen for differentially expressed genes with functions that had been predicted by bioinformatics.

4. The results showed that there were 2416 differentially expressed genes between the two groups, 53 of which were involved in temperature-sensitive pathways. The protein-protein interaction network combined these 53 temperature-sensitive genes and another group of 65 genes, which enriched the development pathway. These results suggested that temperature-sensitive genes may be involved in growth and development related pathways.

1.鸟类的新产卵胚盘在低温储存过程中可以在休眠状态下存活数周。先前的研究表明，鸡胚的临界温度范围为19至27°C。在此范围内，胚胎将在休眠状态下滞育。一旦温度上升到该范围以上，胚盘将打破休眠状态。

2.阐明启动鸭胚从胚盘休眠中恢复发育的机制，将有助于改变温度控制以改善家禽生产中的孵化。假设可能有一些温度敏感基因参与从胚盘休眠中启动鸭胚胎发育恢复。

3.为了检验这一假设，对新产卵的鸭胚盘和鸭胚胎（孵育48小时）的转录组进行测序，以筛选具有生物信息学预测功能的差异表达基因。

4.结果表明，两组之间有2416个差异表达基因，其中53个涉及温度敏感性途径。蛋白质-蛋白质相互作用网络将这53个温度敏感基因和另一组65个基因结合在一起，丰富了发育途径。这些结果表明，温度敏感基因可能参与了与生长和发育相关的途径。