ABSTRACT Cytosolic FBA functions in the sucrose biosynthetic pathway and is also a key enzyme in glycolytic pathways in higher plants. In this paper, a cytoplasmic FBA gene (designated CoFBA1) expressed specifically in the endosperm of coconut was identified and cloned. Prediction analysis suggested that the subcellular location of CoFBA1 was the cytoplasm. Then, CoFBA1 was cloned into the pCAMBIA1300s vector driven by the seed-specific promoter napin to verify the in vivo activity of CoFBA1 in Arabidopsis. Three transgenic lines with different levels of CoFBA1 expression were selected for gene expression, total oil content and fatty acid content analyses. In the three overexpression homozygous transgenic lines, the CoFBA1 gene consistently increased in expression. The lipid content in the three transgenic lines increased by 3.5 – 18% compared to that in WT. The total seed lipid content in the three lines simultaneously exhibited a significant increase (P < 0.01), while no significant difference was detected among the three transgenic lines. Moreover, linolenic acid (C18:3) and eicosanoic acid (C20:1) increased significantly in the transgenic lines. It was suggested that CoFBA1 overexpression not only increased the total lipid content but also changed the composition of the fatty acids in the transgenic lines.

中文翻译：

---

来自椰子（Cocos nucifera L.）的细胞质果糖-1，6-二磷酸醛缩酶基因的分子克隆和功能表征

摘要 胞质 FBA 在蔗糖生物合成途径中起作用，也是高等植物糖酵解途径中的关键酶。在本文中，鉴定并克隆了一种在椰子胚乳中特异性表达的细胞质 FBA 基因（命名为 CoFBA1）。预测分析表明 CoFBA1 的亚细胞位置是细胞质。然后，将CoFBA1克隆到由种子特异性启动子napin驱动的pCAMBIA1300s载体中，以验证CoFBA1在拟南芥中的体内活性。选择具有不同 CoFBA1 表达水平的三个转基因品系进行基因表达、总油含量和脂肪酸含量分析。在三个过表达纯合转基因品系中，CoFBA1 基因的表达持续增加。三个转基因品系的脂质含量增加了3。5 – 18% 与 WT 相比。三个品系的种子总脂质含量同时呈现显着增加（P < 0.01），而三个转基因品系之间没有检测到显着差异。此外，亚麻酸 (C18:3) 和二十烷酸 (C20:1) 在转基因品系中显着增加。表明 CoFBA1 过表达不仅增加了总脂质含量，而且还改变了转基因品系中脂肪酸的组成。