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A comparative study of phytotoxic effects of metal oxide (CuO, ZnO and NiO) nanoparticles on in-vitro grown Abelmoschus esculentus
Plant Biosystems ( IF 2 ) Pub Date : 2020-05-04 , DOI: 10.1080/11263504.2020.1753843
Venkidasamy Baskar 1 , Nayeem Safia 1 , Kuppuraj Sree Preethy 1 , Selvaraj Dhivya 1 , Muthu Thiruvengadam 2 , Ramalingam Sathishkumar 1
Affiliation  

Abstract

The extensive use of nanoparticles (NPs) in various industrial and household products pose a threat of their release into the environment. Plants being sessile are prone to be directly affected by these nanomaterials in polluted soils, which may represent possible risks to food safety and human health. In the present study, we compared the phytotoxic effects of metal oxide NPs namely nickel oxide (NiO), copper oxide (CuO) and zinc oxide (ZnO) NPs at four varying concentrations (100, 250, 500 and 1000 mg/L) on in-vitro grown seedlings of Abelmoschus esculentus (okra). All the NPs tested demonstrated a suppressed plant growth in a concentration-dependent manner which was evidenced from the repression of shoot and root length. A decrease in chlorophyll content was recorded in the NP-treated plants. Anthocyanin contents, total phenolic and total flavonoid contents were significantly altered in the NP-treated plants. Moreover, reactive oxygen species (ROS) generation was monitored in the roots of NP-treated plants using 4-nitro blue tetrazolium chloride (NBT) and 3, 3′-diaminobenzidine (DAB) staining, demonstrated an increase in ROS production in a dose-dependent manner in plants exposed to all three NPs. Enhanced ROS and malondialdehyde (MDA) production was recorded in A. esculentus seedlings when exposed to NPs. Among the three metal oxide NPs compared in the study, NiO NPs were found to be more toxic than CuO and ZnO ones.



中文翻译:

金属氧化物(CuO,ZnO和NiO)纳米粒子对体外培养的Abelmoschus esculentus的植物毒性作用的比较研究

摘要

纳米颗粒(NPs)在各种工业和家庭产品中的广泛使用构成了将其释放到环境中的威胁。在污染的土壤中,易生植物易受这些纳米材料的直接影响,这可能对食品安全和人类健康构成潜在风险。在本研究中,我们比较了四种不同浓度(100、250、500和1000 mg / L)的金属氧化物NPs,即氧化镍(NiO),氧化铜(CuO)和氧化锌(ZnO)NPs的植物毒性作用。番茄Abelmoschus esculentus的离体培养幼苗(秋葵)。测试的所有NP均显示出浓度依赖性的抑制植物生长,这从芽和根长度的抑制得到证明。在NP处理过的植物中记录到叶绿素含量的减少。在NP处理的植物中,花色苷含量,总酚和总类黄酮含量均发生了显着变化。此外,使用4-硝基蓝四唑鎓氯化物(NBT)和3,3'-二氨基联苯胺(DAB)染色监测了NP处理植物的根中的活性氧(ROS)生成,证明一定剂量下ROS产量增加暴露于所有三个NP的植物中的依赖方式。增强的ROS和丙二醛(MDA)的生产录得A.荸荠暴露于NP时的幼苗。在研究中比较的三种金属氧化物NPs中,发现NiO NPs比CuO和ZnO具有更高的毒性。

更新日期:2020-05-04
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