Abstract

In order to obtain a better knowledge about the endangered species Colletia paradoxa (Rhamnaceae), which appears in fragmented populations, a study was carried out with six populations from different physiographic regions of Rio Grande do Sul and Santa Catarina states, Brazil. The objective of this study is to investigate the real conditions of genetic diversity in populations, through the use of inter simple sequence repeat (ISSR) molecular marker, and to evaluate the distribution of genetic diversity among and within populations, in order to plan conservation and management strategies. Five selected primers produced 112 DNA fragments in 46 samples from individuals that were evaluated. The result of the analyses showed 100% polymorphism. Nei’s genetic diversity index (H) was 0.3083. Shannon’s information index (I) was 0.4880. Bayesian inference of clustering performed by Structure revealed the most likely K = 4 to C. paradoxa. The analysis of molecular variance among all populations showed that the greatest genetic diversity occurs within the populations (82%), while among them there is less differentiation (18%), although in some populations good levels of genetic migration occur (Nm = 1.0465) indicating that genetic exchanges among populations of C. paradoxa are still occurring.

摘要

为了更好地了解濒临灭绝的物种Colletia paradoxa（鼠李科）出现在零散的种群中，对来自南里奥格兰德州和巴西圣卡塔琳娜州不同地理区域的六个种群进行了研究。这项研究的目的是通过使用简单序列重复（ISSR）分子标记研究种群遗传多样性的真实条件，并评估种群之间和种群内部遗传多样性的分布，以计划保护和保护工作。管理策略。五个被选择的引物在来自被评估个体的46个样品中产生112个DNA片段。分析结果表明100％多态性。Nei的遗传多样性指数（H）为0.3083。香农的信息指数（I）为0.4880。结构进行聚类的贝叶斯推断表明，最有可能K = 4到C.悖论。所有种群之间的分子变异分析表明，最大的遗传多样性发生在种群内（82％），而其中的分化程度较小（18％），尽管在某些种群中发生了良好的遗传迁移水平（Nm = 1.0465）这表明，C。paradoxa种群之间的遗传交流仍在发生。