Abstract

Cistus species are widespread in the Mediterranean basin, especially in places characterized by high temperatures and arid soils. Despite a rich literature concerning the biological and pharmacological properties of Cistus species, to our knowledge, there are only few reports on pollen viability and little is known about microbial endophytes within pollen grains. Pollen viability and germination of Cistus creticus L. and C. monspeliensis L., from two sites of Sicily (Italy) over two years (2016–2017), were determined by 2,3,5 triphenyl-tetrazolium chloride (TTC) staining and an in vitro germination test. Dominant microorganisms, flowing from pollen openings (colpora), were identified by molecular methods. Furthermore, for comparison, in 2017 microbial endophytes of C. eriocephalus Viv. from Serra S. Bruno (Calabria, Italy) were studied. The optimal medium composition for rapid in vitro germination was determined. Pollen viability was in the range of 79.8–90.0% for C. monspeliensis and 86.6–91.6% for C. creticus, while germination was 56.5–62.4% for C. monspeliensis and 50.1–58.8 for C. creticus. Statistical analysis by correlation coefficient showed a significant relationship between viability and germination, whereas significant differences (P < 0.05) were observed between TTC staining and the in vitro germination test. Yeasts were the dominant microorganisms in all pollen samples and dominant species were identified.

摘要

水犀科物种广泛分布在地中海盆地，特别是在以高温和干旱土壤为特征的地区。尽管有大量有关水犀科物种生物学和药理特性的文献，据我们所知，关于花粉生存力的报道很少，对花粉粒中微生物内生菌的了解甚少。通过2,3,5三苯基四唑鎓氯化物（TTC）染色确定了西西里岛（意大利）的两个地点在过去两年（2016-2017年）的Cistus creticus L.和C. monspeliensis L.的花粉生存力和发芽。的体外发芽测试。从花粉开口（colpora）流出的主要微生物已通过分子方法鉴定。此外，为了进行比较，2017年C.eriocephalus Viv的微生物内生菌。来自Serra S. Bruno（意大利Calabria）的研究。确定了用于快速体外萌发的最佳培养基组成。花粉活力是为79.8-90.0％的范围内C. monspeliensis和86.6-91.6％C. creticus，而发芽是为56.5-62.4％C. monspeliensis用于和50.1-58.8 C. creticus。通过相关系数进行的统计分析表明，活力与发芽率之间存在显着相关性，而TTC染色与体外发芽试验之间存在显着差异（P <0.05）。酵母是所有花粉样品中的优势微生物，并且已鉴定出优势物种。