ABSTRACTThe natural environment of Geobacter sulfurreducens is oligotrophic and described as limiting in both electron donors and terminal electron acceptors (TEA). In previous studies we examined the effects of long-term TEA (fumarate) limitation, and in this study we examine long-term batch cultures under limiting electron donor. The microorganism survived under long-term electron donor (acetate) starvation, maintaining a stable population of ∼1–2× 108 cells mL−1for >650 days. Proteins that varied in abundance with a high level of statistical significance (p < 0.05) for stages between mid-log to survival phase (acetate starved) were identified using iTRAQ based mass spectroscopy. The most highly represented proteins that significantly increased in level in the survival phase cells are generally membrane-associated and are involved in energy metabolism and protein fate. These results document that changes in the outer and cytoplasmic membranes may help G. sulfurreducens survive during starvation through ...

中文翻译：

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长期供电子饥饿条件下硫还原土杆菌PCA的表征和蛋白质组学分析

摘要硫还原地杆菌的自然环境是贫营养的，被描述为限制电子供体和末端电子受体 (TEA)。在以前的研究中，我们检查了长期 TEA（富马酸盐）限制的影响，在本研究中，我们检查了限制电子供体下的长期分批培养。微生物在长期电子供体（乙酸盐）饥饿下存活，维持~1-2×108 个细胞 mL-1 的稳定种群>650 天。使用基于 iTRAQ 的质谱法鉴定了在对数中期到存活期（乙酸盐缺乏）之间的阶段，丰度变化具有高水平统计显着性（p < 0.05）的蛋白质。在存活期细胞中水平显着增加的最具代表性的蛋白质通常与膜相关并参与能量代谢和蛋白质命运。这些结果表明，外膜和细胞质膜的变化可能有助于 G.sulfurreducens 在饥饿期间通过……