ABSTRACT

With the increase in the cognizance toward the wide and abundant lignocellulosic biomass, a great interest has been garnered toward the production of value-added products from the biomass. Hence, by capitalizing the Casuarina equisetifolia biomass, the current work developed a simultaneous pre-treatment and saccharification (SPS) process using transgenic Trichoderma atroviride. The ability of T. atroviride to produce lignolytic and cellulolytic enzymes was enhanced by optimizing key process parameters. Under the optimized conditions, a maximum of 1245.6 U/kg of cellulase and 1203.36 U/kg of xylanase, 183.2 U/kg of laccase along with 392.36 g/kg of fermentable sugars were obtained. On comparing with acid and alkaline pre-treatment methods, the T. atroviride -mediated SPS process exhibited trace formation of fermentative inhibitors, which resulted in a minimal inhibition of Escherichia coli. Overall, the current work implements the biorefinery concept on Casuarina equisetifolia biomass by advocating circular economy.

Implications: Valorization of lignocellulosic waste biomass into value added compound and as biofuel is considered as a promising alternative resource, owing to its availability and low production cost. However, the presence of chemically resistant lignin demands an intensive treatment process, which sometimes leads to the formation of fermentative inhibitors. Casuarina equisetifolia is a deciduous commercial plant, and an average of 125 tonnes/hector of waste is generated annually in India. By considering the demerit of delignification and the wide availability of Casuarina equisetifolia biomass (CB), the current work aimed at the development of a single-pot simultaneous pre-treatment and saccharification (SPS) of CB by transgenic Trichoderma atroviride.

摘要

随着对广泛而丰富的木质纤维素生物质的认识的提高，人们开始对利用生物质生产增值产品产生了极大的兴趣。因此，通过利用木麻黄（Casuarina equisetifolia）生物质进行资本化，目前的工作开发了使用转基因木霉木霉（Atroviride）的同时预处理和糖化（SPS）工艺。的能力T. atrovirid e，以产生木质素降解和纤维素分解酶通过优化关键工艺参数增强。在优化的条件下，纤维素酶最大量为1245.6 U / kg，木聚糖酶最大量为1203.36 U / kg，漆酶最大量为183.2 U / kg，可发酵糖量为392.36 g / kg。与酸和碱预处理方法相比，T. atrovirid e介导的SPS过程显示出痕量的发酵抑制剂，这导致对大肠杆菌的抑制作用最小。总体而言，当前工作通过倡导循环经济在木麻黄上实现了生物精炼概念。

含义：将木质纤维素废料生物量增值成增值化合物，并作为生物燃料，由于其可获得性和低生产成本而被认为是一种有前途的替代资源。但是，化学抗性木质素的存在需要密集的处理过程，有时会导致形成发酵抑制剂。木麻黄（Casuarina equisetifolia）是一种落叶商业植物，印度每年平均产生125吨/公顷的废物。考虑到脱木素的缺点和木麻黄生物量（CB）的广泛可用性，当前的工作旨在通过转基因阿氏木霉菌开发CB的单锅同时预处理和糖化（SPS）。