Abstract

Objective

To explore the relationship between cerebrospinal fluid-contacting neurons (CSF-cNs) and endogenous neural progenitor cells (ENPCs) and whether CSF-cNs are involved in nerve repair after spinal cord injury (SCI).

Methods

Cholera toxin B-horseradish peroxidase complex (CB-HRP) and cholera toxin B conjugated with saporin (CB-SAP) were injected into the lateral ventricles of spinal cord injured rats to mark and destroy the CSF-cNs. Then the rats in the experimental group were injured by SCI. Observe the content and co-expression of CSF-cNs and ENPCs in rats of each group, and observe the recovery of motor function after SCI in each group.

Results

After the destruction of CSF-cNs, the number of ENPCs decreased significantly in the long term after the surgery, and the recovery of motor function also deteriorated as compared to the group with intact CSF-cNs. Meanwhile some cells in the spinal cord express both the biological marker of CSF-cNs and ENPCs.

Conclusion

This study shows that the population of ENPCs and motor function recovery in SCI rats declined after the destruction of CSF-cNs, suggesting that CSF-cNs affect the ENPCs population and may be involved in the recovery of neural function after SCI.

中文翻译：

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脑脊液接触神经元影响脊髓损伤后内源性神经祖细胞的表达和神经功能的恢复

摘要

客观的

探讨脑脊液接触神经元（CSF-cNs）和内源性神经祖细胞（ENPCs）之间的关系，以及脊髓损伤（SCI）后CSF-cNs是否参与神经修复。

方法

将霍乱毒素B-辣根过氧化物酶复合物（CB-HRP）和与皂素结合的霍乱毒素B（CB-SAP）注入脊髓损伤大鼠的侧脑室以标记和破坏CSF-cNs。然后实验组大鼠被脊髓损伤。观察各组大鼠CSF-cNs和ENPCs的含量和共表达，观察各组SCI后运动功能的恢复情况。

结果

CSF-cNs破坏后，与术后完整的CSF-cNs组相比，术后长期内ENPC的数量显着减少，运动功能的恢复也恶化。同时，脊髓中的某些细胞同时表达CSF-cNs和ENPCs的生物学标记。

结论

这项研究表明，CSF-cNs破坏后，SCI大鼠的ENPC数量和运动功能恢复下降，这表明CSF-cNs影响ENPCs数量，并可能参与SCI后的神经功能恢复。