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Tripartite motif containing 59 (TRIM59) promotes esophageal cancer progression via promoting MST4 expression and ERK pathway
Journal of Receptors and Signal Transduction ( IF 2.8 ) Pub Date : 2020-04-27 , DOI: 10.1080/10799893.2020.1756327
Guangming Liu 1 , Jinying Song 2 , Yong Zhao 2 , Lianjie Zhang 2 , Junjie Qin 1 , Youbin Cui 2
Affiliation  

Abstract Objective: To detect the expression of tripartite motif containing 59 (TRIM59) in human esophageal cancer (EC) tissues and explore whether TRIM59 could affect the progression of EC. Methods: Quantitative PCR and immunohistochemistry assays were performed to detect the expression of TRIM59 in 40 human EC tissues and corresponding non-tumor tissues. The correlations between TRIM59 expression and clinical pathological features of patients with EC were also investigated. CCK-8, colony formation, wound closure, and transwell assays were performed to detect the effects of TRIM59 on EC cells in vitro., Immunoblotting assays were performed to detect the effects of TRIM59 on the expression of mammalian sterile-20-like kinase 4 (MST4) and ERK pathway. Results: We reported increased expression of TRIM59 in human EC tissues, and its expression was correlated with clinical features, including metastasis (p = .011*) and maximum diameter (p = .027*), in patients with EC. We further found that TRIM59 contributed to the proliferation and invasion of EC cells via regulating mammalian sterile-20-like kinase 4 (MST4) expression and ERK pathway. Conclusion: Our data confirmed the involvement of TRIM59 in EC progression and proposed that TRIM59 could serve as a promising therapeutic target for the treatment of EC.

中文翻译:

含有 59 (TRIM59) 的三方基序通过促进 MST4 表达和 ERK 通路促进食管癌进展

摘要 目的:检测人食管癌(EC)组织中含有59的三联基序(TRIM59)的表达,探讨TRIM59是否影响EC的进展。方法:采用定量 PCR 和免疫组化方法检测 TRIM59 在 40 份人 EC 组织和相应的非肿瘤组织中的表达。还研究了 TRIM59 表达与 EC 患者临床病理特征之间的相关性。进行 CCK-8、集落形成、伤口闭合和 Transwell 试验以检测 TRIM59 对体外 EC 细胞的影响。进行免疫印迹试验以检测 TRIM59 对哺乳动物无菌 20 样激酶 4 表达的影响(MST4) 和 ERK 途径。结果:我们报告了 TRIM59 在人 EC 组织中的表达增加,并且其表达与 EC 患者的临床特征相关,包括转移 (p = .011*) 和最大直径 (p = .027*)。我们进一步发现TRIM59通过调节哺乳动物不育20样激酶4(MST4)表达和ERK通路促进EC细胞的增殖和侵袭。结论:我们的数据证实了 TRIM59 参与 EC 进展,并提出 TRIM59 可以作为治疗 EC 的有希望的治疗靶点。
更新日期:2020-04-27
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