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Exendin-4 attenuates inflammation-mediated endothelial cell apoptosis in varicose veins through inhibiting the MAPK-JNK signaling pathway
Journal of Receptors and Signal Transduction ( IF 2.8 ) Pub Date : 2020-04-25 , DOI: 10.1080/10799893.2020.1756326
Changfu Hu 1 , Kai Lu 2 , Weili Liu 2
Affiliation  

Abstract Context: Inflammation response has been found to be associated with endothelial cell death in the progression of varicose veins. Exendin-4 is able to reduce inflammation and thus attenuate cell apoptosis. Aim: The aim of our study is to explore the influence of Exendin-4 on LPS-treated endothelial cells. Methods: Cells were treated with LPS. Exendin-4 was added into the medium of cells. Western blots, qPCR, and ELISA were used to analyze the role of Exendin-4 in LPS-mediated cell death. Results: We found that LPS treatment caused significantly cell death. Whereas this trend could be attenuated by Exendin-4. After treatment with Exendin-4, inflammation factors upregulation and oxidative stress activation were significantly repressed, an effect that was followed by a drop in the levels of glucose production and lactic acid generation. At the molecular levels, Exendin-4 treatment inhibited the activity of MAPK-JNK signaling pathway in the presence of LPS treatment. Conclusions: LPS causes cell apoptosis through inducing inflammation response, oxidative stress and energy stress. Exendin-4 treatment enhances cell survival, reduces inflammation, and improves energy stress through inhibiting the MAPK-JNK signaling pathway.

中文翻译:

Exendin-4通过抑制MAPK-JNK信号通路减轻静脉曲张炎症介导的内皮细胞凋亡

摘要背景:已发现炎症反应与静脉曲张进展中的内皮细胞死亡有关。Exendin-4 能够减少炎症,从而减轻细胞凋亡。目的:我们研究的目的是探讨 Exendin-4 对 LPS 处理的内皮细胞的影响。方法:用LPS处理细胞。将Exendin-4加入细胞培养基中。蛋白质印迹、qPCR 和 ELISA 用于分析 Exendin-4 在 LPS 介导的细胞死亡中的作用。结果:我们发现 LPS 处理导致显着的细胞死亡。而这种趋势可能会被 Exendin-4 减弱。用 Exendin-4 治疗后,炎症因子上调和氧化应激激活受到显着抑制,随后葡萄糖生成和乳酸生成水平下降。在分子水平上,Exendin-4 处理在 LPS 处理存在下抑制了 MAPK-JNK 信号通路的活性。结论:LPS通过诱导炎症反应、氧化应激和能量应激引起细胞凋亡。Exendin-4 治疗通过抑制 MAPK-JNK 信号通路增强细胞存活、减少炎症并改善能量应激。
更新日期:2020-04-25
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