Abstract Dynamin-related protein-1 (Drp1) has been found to be associated with cell death. The role of Drp1 in A549 cells death has not been explored. In this study, adenovirus-mediated Drp1 overexpression was used to investigate the influence of Drp1 on A549 cell viability with a focus on F-actin and Bax. Cell viability, protein expression, oxygen consumption, energy metabolism, and growth rate were measured through ELISA, qPCR, western blots and pathway analysis. Our results indicated that Drp1 overexpression promoted A549 cell death through apoptosis. Mechanistically, cytoskeletal F-actin was impaired and Bax expression was elevated in response to Drp1 overexpression. Besides, energy metabolism was reduced and oxygen consumption was interrupted. Therefore, our results demonstrated that A549 cell viability, apoptosis and growth were regulated by the Drp1/F-actin/Bax signaling pathways. These data explain a new role played by Drp1 in regulating cell viability and also provide a potential target to affect the progression of lung cancer through induction of cell death.

中文翻译：

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Dynamin相关蛋白-1通过F-actin/bax信号通路促进肺癌A549细胞凋亡

摘要 Dynamin 相关蛋白-1 (Drp1) 已被发现与细胞死亡有关。尚未探索 Drp1 在 A549 细胞死亡中的作用。在这项研究中，腺病毒介导的 Drp1 过表达用于研究 Drp1 对 A549 细胞活力的影响，重点是 F-肌动蛋白和 Bax。通过ELISA、qPCR、蛋白质印迹和通路分析测量细胞活力、蛋白质表达、耗氧量、能量代谢和生长速率。我们的结果表明，Drp1 过表达通过细胞凋亡促进 A549 细胞死亡。从机制上讲，细胞骨架 F-肌动蛋白受损，Bax 表达升高以响应 Drp1 过表达。此外，能量代谢减少，耗氧量中断。因此，我们的结果表明 A549 细胞活力，细胞凋亡和生长受 Drp1/F-肌动蛋白/Bax 信号通路调节。这些数据解释了 Drp1 在调节细胞活力方面的新作用，并提供了一个潜在的靶点，通过诱导细胞死亡来影响肺癌的进展。