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Interaction of the mTERT telomerase catalytic subunit with the c-Abl tyrosine kinase in mouse granulosa cells
Journal of Receptors and Signal Transduction ( IF 2.8 ) Pub Date : 2020-03-04 , DOI: 10.1080/10799893.2020.1735419
Aylin Yaba 1 , Sami Agus 2 , Ecem Yıldırım 1 , Cihan Süleyman Erdogan 2 , Bayram Yılmaz 2
Affiliation  

Abstract Context: Oocyte and granulosa cells (GCs) have bidirectional communication and GCs play an important role in folliculogenesis and proliferation of GCs is very important for the development of ovulatory follicle. DNA double-strand breaks activate c-Abl protein tyrosine kinase and c-Abl has a functional role in repairement of DNA and control of telomere. Objective: In this study, we hypothesized that c-Abl has a regulative role on mTERT in mouse ovarian granulosa cells (GCs) and we aimed to detect c-Abl and mTERT interaction in mouse primary culture of GCs. Materials and methods: Mouse ovarian granulosa cell were cultured and siRNA-mediated knockdown approach was used to knockdown c-Abl expression. Results: We showed c-Abl and mTERT immunolocalization in vivo and in vitro mouse GCs. c-Abl and mTERT were constitutively expressed in mouse granulosa cells and c-Abl presented more intense expression in granulosa cells than mTERT expression. The interaction of the c-Abl-mTERT is supported by the exhibition that c-Abl siRNA knockdown cells show decreased mTERT expression. We also present an interaction between c-Abl and mTERT by immunoprecipitation. In addition, our results indicated that the down-regulation of c-Abl was also accompanied by reduced expression of proliferating cell nuclear antigen (PCNA) in GCs. Conclusions: We suggest that mTERT may associate with the c-Abl in mouse GCs and the interactions between c-Abl and mTERT suggest a role for c-Abl in the regulation of telomerase function and proliferation in mouse granulosa cells.

中文翻译:

mTERT 端粒酶催化亚基与小鼠颗粒细胞中 c-Abl 酪氨酸激酶的相互作用

摘要背景:卵母细胞和颗粒细胞(GCs)具有双向通讯功能,GCs 在卵泡发生中起重要作用,GCs 的增殖对排卵卵泡的发育非常重要。DNA双链断裂激活c-Abl蛋白酪氨酸激酶,c-Abl在DNA修复和端粒控制中具有功能性作用。目的:在本研究中,我们假设 c-Abl 对小鼠卵巢颗粒细胞 (GCs) 中的 mTERT 具有调节作用,我们旨在检测小鼠 GCs 原代培养中 c-Abl 和 mTERT 的相互作用。材料与方法:培养小鼠卵巢颗粒细胞,采用siRNA介导的敲低方法敲低c-Abl表达。结果:我们在体内和体外小鼠 GC 中显示了 c-Abl 和 mTERT 免疫定位。c-Abl 和 mTERT 在小鼠颗粒细胞中组成型表达,c-Abl 在颗粒细胞中的表达比 mTERT 表达更强烈。c-Abl-mTERT 的相互作用得到了 c-Abl siRNA 敲低细胞显示降低的 mTERT 表达的支持。我们还通过免疫沉淀介绍了 c-Abl 和 mTERT 之间的相互作用。此外,我们的结果表明,c-Abl 的下调还伴随着 GC 中增殖细胞核抗原 (PCNA) 的表达降低。结论:我们认为 mTERT 可能与小鼠 GC 中的 c-Abl 相关,c-Abl 和 mTERT 之间的相互作用表明 c-Abl 在调节小鼠颗粒细胞中端粒酶功能和增殖中的作用。
更新日期:2020-03-04
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