In this study, a method was established for discriminating the true Cervus antlers from its counterfeits using TaqMan real-time quantitative PCR. The method combines the use of true Cervus antlers-specific primers, that amplify a 226 bp fragment from true Cervus antlers DNA, and mammalian-specific primers amplifying a 146 bp fragment from mammalian species DNA, which are used as endogenous control. A TaqMan probe that hybridizes in the ‘Cervus antler’ and also in the ‘mammalian’ DNA fragments is used to monitor the amplification of the target gene. The Cervus antler mitochondrial DNA was used as target gene to design the primers and TaqMan probes. The data revealed that the TaqMan real-time PCR-based assay can be used for identification of the true Cervus antlers from counterfeits in a single step. The limit of detection (LOD) was lower than 1 pg of DNA per reaction.

在这项研究中，建立了识别真正的方法鹿使用TaqMan实时定量PCR的假冒鹿角。该方法结合使用的真鹿鹿角特异性引物，即从真实扩增226 bp的片段鹿鹿角DNA和哺乳动物特异性引物扩增来自哺乳动物物种的DNA，被用作内源性对照一个146 bp的片段。TaqMan探针在“杂交的鹿鹿茸”以及在“哺乳动物” DNA片段是用来监视靶基因的扩增。在鹿以鹿角线粒体DNA为靶基因设计引物和TaqMan探针。该数据表明，TaqMan TM实时PCR为基础的试验可用于识别真实的鹿在单个步骤中从假冒鹿角。每个反应的检出限（LOD）低于1 pg DNA。