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A novel mitochondria-targeting method using special staining for the detection of apoptotic hepatocytes
Journal of Histotechnology ( IF 1.1 ) Pub Date : 2020-03-13 , DOI: 10.1080/01478885.2020.1721767
Adrian Florin Gal 1 , Flavia Ruxanda 1 , Vasile Rus 1 , Sanda Andrei 2 , Viorel Miclăuş 1
Affiliation  

ABSTRACT Early detection of apoptotic cells on histological slides is of major importance for both diagnostic and research areas. In the current study, the aim was to propose a convenient method to stain the mitochondria and establish whether hepatocytes undergoing apoptosis can be identified in tissue sections using the proposed method. Liver tissue from five adult chinchillas was fixed with 10% neutral buffered formalin for Goldner’s trichrome (GT) and Groat’s iron hematoxylin and eosin (HE) stains and with Kolster’s fixative for the Heidenhain’s iron hematoxylin procedure. The HE and GT-stained sections showed the morphological features consistent with apoptosis i.e., homogenous intensely acidophilic cytoplasm, cell shrinkage with an irregular outline, nuclear shrinkage with cloudy karyoplasm, and karyopyknosis in the late stage. Sections stained with Heidenhain’s iron hematoxylin method was used to pinpoint mitochondria and revealed cells which were undergoing the first stages of the apoptosis process i.e., disappearance of mitochondria from the cell, chromatin condensation and margination, paracentral localization of nucleoli, and vacuolated nuclei. In more advanced stages of apoptosis, cells presented significant nuclear and cytoplasmic changes. It was concluded that this is the first report targeting the mitochondria, by performing inexpensive histological staining techniques, in order to assess dead cells in situ.

中文翻译:

一种使用特殊染色检测凋亡肝细胞的线粒体靶向新方法

摘要 组织学载玻片上凋亡细胞的早期检测对于诊断和研究领域都具有重要意义。在目前的研究中,目的是提出一种方便的方法来染色线粒体,并确定是否可以使用所提出的方法在组织切片中鉴定发生凋亡的肝细胞。来自五只成年龙猫的肝脏组织用 10% 中性缓冲福尔马林固定,用于 Goldner 三色 (GT) 和 Groat 铁苏木精和伊红 (HE) 染色,并用 Kolster 固定剂固定 Heidenhain 铁苏木精程序。HE和GT染色切片显示出与细胞凋亡一致的形态特征,即细胞质均一的强嗜酸性,细胞皱缩,轮廓不规则,核皱缩,核质混浊,晚期核固缩。使用 Heidenhain 铁苏木精法染色的切片来精确定位线粒体,并揭示正在经历凋亡过程第一阶段的细胞,即线粒体从细胞中消失、染色质浓缩和边缘化、核仁的旁中心定位和空泡化的细胞核。在细胞凋亡的更高级阶段,细胞呈现出显着的核和细胞质变化。得出的结论是,这是第一份针对线粒体的报告,通过执行廉价的组织学染色技术,以原位评估死细胞。核仁的中央定位和空泡化的核。在细胞凋亡的更高级阶段,细胞呈现出显着的核和细胞质变化。得出的结论是,这是第一份针对线粒体的报告,通过执行廉价的组织学染色技术,以原位评估死细胞。核仁的中央定位和空泡化的核。在细胞凋亡的更高级阶段,细胞呈现出显着的核和细胞质变化。得出的结论是,这是第一份针对线粒体的报告,通过执行廉价的组织学染色技术,以原位评估死细胞。
更新日期:2020-03-13
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