Abstract The postmortem diagnosis of fatal heatstroke (hyperthermia) is a challenging task in forensic practice. Attenuated Total Reflectance Fourier transform infrared spectroscopy combined with chemometrics was utilized to explore the biochemical difference of fatal heatstroke and to establish diagnosis models in this study. The complementary results of spectral absorbance comparison and principal component analysis demonstrated that the spectral differences between the hyperthermia and control groups mainly arose from the variations in the structure or composition of nucleic acids, proteins, amino acids, and carbohydrates in the vitreous humor. A genetic algorithm-guided partial least squares discriminant analysis model was established for the postmortem diagnosis of heatstroke, which achieved great specificity and sensitivity, both with scores of 100%. Furthermore, classification predictions of unknown vitreous humor samples collected from 11 rabbits were also performed by the model, resulting in 100% correct discrimination. This preliminary research demonstrates that Attenuated Total Reflectance Fourier transform infrared spectroscopy in combination with chemometrics has the potential to be an effective aid for the postmortem diagnosis of fatal heatstroke.

中文翻译：

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使用衰减全反射傅里叶变换红外光谱结合化学计量学表征和死后诊断致命性中暑

摘要 致命中暑（体温过高）的死后诊断是法医实践中的一项具有挑战性的任务。本研究利用衰减全反射傅里叶变换红外光谱结合化学计量学来探讨致死性中暑的生化差异并建立诊断模型。光谱吸光度比较和主成分分析的互补结果表明，热疗组和对照组之间的光谱差异主要来自玻璃体液中核酸、蛋白质、氨基酸和碳水化合物的结构或组成的变化。建立了遗传算法引导的偏最小二乘判别分析模型用于中暑的尸检诊断，具有很高的特异性和敏感性，两者的得分均为 100%。此外，该模型还对采集自 11 只兔子的未知玻璃体液样本进行了分类预测，实现了 100% 的正确判别。这项初步研究表明，衰减全反射傅里叶变换红外光谱与化学计量学相结合有可能成为致命中暑的尸检诊断的有效辅助手段。