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An Overview of Proteases: Production, Downstream Processes and Industrial Applications
Separation and Purification Reviews ( IF 5.4 ) Pub Date : 2019-10-19 , DOI: 10.1080/15422119.2019.1677249
Nathiele Contrera Gimenes 1 , Edgar Silveira 2, 3 , Elias Basile Tambourgi 1
Affiliation  

Peptidases or proteases are enzymes with the ability to break peptide bonds. They have been investigated due to their industrial applications in products such as foods and detergents. This review provides an update of protease characteristics, production, downstream processes and industrial applications. Proteases are predominantly produced by microorganisms using submerged and solid-state fermentations. The bottleneck of production is found in downstream processes, which can account for 70 to 90% of the total production cost. Precipitation is used in addition to extraction using aqueous two-phase systems (ATPSs). ATPS extraction has emerged as a recovery method enabling to clarify, to concentrate, and to purify the target biomolecule in a single operation. However, efficient cost-effective strategies need to be improved in order to provide peptidases to industries on a sufficient level. The search for new protease sources has been encouraged in order to meet industrial application requirements.



中文翻译:

蛋白酶概述:生产,下游工艺和工业应用

肽酶或蛋白酶是具有断裂肽键能力的酶。由于它们在食品和洗涤剂等产品中的工业应用,已对其进行了研究。这篇评论提供了蛋白酶特性,生产,下游工艺和工业应用的更新。蛋白酶主要由微生物使用浸没式和固态发酵产生。生产瓶颈存在于下游工艺中,占总生产成本的70%至90%。除使用水相两相系统(ATPSs)萃取外,还会使用沉淀。ATPS提取已成为一种回收方法,可在一次操作中澄清,浓缩和纯化目标生物分子。然而,为了向工业提供足够水平的肽酶,需要改进有效的,具有成本效益的策略。为了满足工业应用需求,已经鼓励寻找新的蛋白酶来源。

更新日期:2019-10-19
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