Protein sample preparation is the most critical step in protein analysis of complex samples and is constituted by tedious, time-consuming, and difficult-to-automate steps that usually involve the use of high volumes of solvents. In recent years, novel extraction or digestion nanomaterials (NMs) have been developed aiming to overcome these drawbacks. In this review, we have grouped the recent works related to the development of new NMs and their applications to the extraction, enrichment/purification, and digestion of proteins. This paper evaluates the role of different kinds of NMs in each step of protein sample preparation focusing on the type of established interaction between the protein and the nanomaterial, their sensitivity and selectivity, their adsorption capacity, and the advantages that they bring in relation to time, efficiency, or reusability.

蛋白质样品的制备是复杂样品蛋白质分析中最关键的步骤，由繁琐，耗时且难以自动化的步骤构成，这些步骤通常涉及使用大量溶剂。近年来，已经开发出新颖的提取或消化纳米材料（NMs）以克服这些缺点。在这篇综述中，我们将与新NM的发展及其在蛋白质的提取，富集/纯化和消化中的应用有关的最新工作归为一组。本文评估蛋白质样品制备各个步骤中不同种类NM的作用，着重于蛋白质与纳米材料之间已建立的相互作用的类型，其敏感性和选择性，吸附能力以及它们带来的时间优势。 ，效率，