Noncoding RNA (ncRNA) genes produce transcripts involved in a wide range of functions, including catalytic and regulatory functions. Besides, some transcripts have highly complex structures that may impact their activities. Among the largest bacterial ncRNAs, there is the rare GOLLD RNA, which is associated with tRNA genes and supposed to be chromosome- and phage-encoded in specialized groups of bacteria, including those from Lactobacillales and Actinomycetales orders. The only GOLLD structure was inferred from a variety of sequences, including many marine metagenomes. To explore GOLLD RNA in bacterial genomes, we mined the GOLLD gene in thousands of Mycobacterium and virus genomes using Infernal software. We identified this gene in 350 mycobacteria, including megaplasmids, and 39 bacteriophages, mainly in the genomic context of tRNA arrays. Mycobacterium GOLLD genes presented a high diversity and were distributed in three phylogenetic groups: (i) Mycobacterium exclusive; (ii) Mycobacterium and mycobacteriophages; and (iii) mycobacteriophage exclusive. We also determined the GOLLD secondary structure of each group using R2 R software based on GOLLD alignments generated by Infernal software. All GOLLD groups displayed a 3ʹ half conserved structure, including utter E-loops pseudoknots substructures, also shared by non-Mycobacterium GOLLD while the 5ʹ half motif was different among the groups. Here, we showed that the lncRNA GOLLD is widespread in Mycobacterium within tRNA arrays and corroborated the previously predicted GOLLD secondary structure.

非编码RNA（ncRNA）基因产生的转录本涉及多种功能，包括催化和调节功能。此外，某些笔录的结构非常复杂，可能会影响其活动。在最大的细菌ncRNA中，有罕见的GOLLD RNA，它与tRNA基因相关，并应该在特殊的细菌群中包括染色体和噬菌体编码，包括来自乳杆菌和放线菌的细菌。唯一的GOLLD结构是从各种序列（包括许多海洋基因组）推论得出的。为了探索细菌基因组中的GOLLD RNA，我们在成千上万的分枝杆菌中挖掘了GOLLD基因和病毒基因组使用Infernal软件。我们在350个分枝杆菌中鉴定了该基因，其中包括大质粒和39个噬菌体，主要在tRNA阵列的基因组背景下。结核分枝GOLLD基因呈现的高多样性并分布在三个亲缘组：（ⅰ）的分枝杆菌排斥; （ii）分枝杆菌和分枝杆菌噬菌体；（iii）分枝杆菌噬菌体除外。我们还基于Infernal软件生成的GOLLD序列，使用R2 R软件确定了每个组的GOLLD二级结构。所有GOLLD组均显示3个半保守结构，包括完全由E型环组成的假结亚结构，也由非分枝杆菌所共有GOLLD的5个半个基序在组中有所不同。在这里，我们表明lncRNA GOLLD在tRNA阵列内的分枝杆菌中广泛分布，并证实了先前预测的GOLLD二级结构。