R-2-(4-hydroxyphenoxy)propionic acid (R-HPPA) is a key chiral intermediate for phenoxypropionic acid herbicide synthesis. In this study, to improve the production of R-HPPA with B. bassiana ZJB16007, the cultivation conditions in solid-state fermentation (SSF) were investigated. The effects of various substrates on R-HPPA production were evaluated and the process parameters were also optimized. The results showed that rice bran was the optimal substrate for R-HPPA production. The optimal medium components and cultivation conditions were: rice bran: silkworm chrysalis powder = 5.25: 2.25 (g: g), nutrient salts solution 12 mL which contained 50 g/L R-PPA, pH 5.0, and cultivated at 28 °C for 11 days. Under the optimized conditions, the transformation of R-HPPA was significantly improved and the yield of R-HPPA reached 77.78%, which was 15.14% higher than that of the control (67.55%). Therefore, SSF may serve as an alternative for R-HPPA production by B. bassiana ZJB16007.

R -2-（4-羟基苯氧基）丙酸（R -HPPA）是苯氧基丙酸除草剂合成的关键手性中间体。在这项研究中，为提高球芽孢杆菌ZJB16007的R -HPPA产量，研究了固态发酵（SSF）的培养条件。评估了各种底物对R -HPPA生产的影响，并优化了工艺参数。结果表明，米糠是生产R -HPPA的最佳基质。最佳培养基成分和培养条件为：米糠：蚕粉= 5.25：2.25（g：g），营养盐溶液12 mL，含50 g / L R-PPA，pH 5.0，并在28°C下培养11天。在优化条件下，R -HPPA的转化率明显提高，R -HPPA的收率达到77.78％，比对照（67.55％）提高了15.14％。因此，SSF可作为一种替代ř通过-HPPA生产白僵菌ZJB16007。