Abstract The study of the mechanism of endogenous microbial flooding in the reservoir, this paper analyzed the effect of microbial and its product on the stability of paraffin wax using Geobacillus stearothermophilu isolated from wells. GC-MS was adopted to investigate the degradation of crude oil by emulsified bacteria. A simulated reservoir environment created by micro-visualized two-dimensional glass model was used to identify mechanism of emulsified bacteria on remaining oil. Emulsified bacteria and their metabolite can greatly improve the efficiency of displacement process. Microorganisms can use crude oil and activator as the source of nutrition, facilitating its grow at the oil-water interface, thus creating a favorable wettability by means of degradation of residual oil. Emulsified bacteria and their metabolite can emulsify the crude oil into emulsion, whose large viscosity attribute to a lower oil-water interface force as well as an improved mobility ratio and in the end, an enlarged swept volume.

中文翻译：

---

嗜热脂肪土芽孢杆菌对原油的影响机制

摘要 研究了油藏内源微生物驱油机理，利用从井中分离得到的嗜热脂肪地芽孢杆菌，分析了微生物及其产物对石蜡稳定性的影响。采用GC-MS研究乳化菌对原油的降解作用。利用微可视化二维玻璃模型创建的模拟储层环境，识别乳化菌对剩余油的作用机理。乳化菌及其代谢产物可以大大提高置换过程的效率。微生物可以利用原油和活化剂作为营养来源，促进其在油水界面生长，从而通过降解残油产生良好的润湿性。