Abstract A facile, straightforward, reliable, and an efficient chemical synthesis of inosine nucleotides such as 7-methylinosine 5′-O-monophosphate, 7-methylinosine 5′-O-diphosphate, and 7-methylinosine 5′-O-triphosphate, starting from the corresponding inosine nucleotide is delineated. The present methylation reaction of inosine nucleotide utilizes dimethyl sulfate as a methylating agent and water as a solvent at room temperature. It is noteworthy that the present methylation reaction proceeds smoothly under aqueous conditions that is highly regioselective to afford exclusive 7-methylinosine nucleotide in good yields with high purity (>99.5%).

中文翻译：

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肌苷核苷酸的高度区域选择性甲基化：7-甲基肌苷核苷酸的有效合成

摘要 一种简便、直接、可靠且有效的肌苷核苷酸化学合成方法，例如 7-甲基肌苷 5'-O-单磷酸、7-甲基肌苷 5'-O-二磷酸和 7-甲基肌苷 5'-O-三磷酸，起始从相应的肌苷核苷酸被划定。本发明的肌苷核苷酸甲基化反应在室温下以硫酸二甲酯为甲基化剂，水为溶剂。值得注意的是，目前的甲基化反应在高度区域选择性的水性条件下顺利进行，以高产率和高纯度（> 99.5%）提供专属的 7-甲基肌苷核苷酸。