Abstract Aerial parts of Plectranthus amboinicus (Lour.) were collected in the Northern Mexico City for a comprehensive effect-directed profiling. Its hydrodistilled P. amboinicus essential oil (PAEO) was separated on HPTLC silica gel plates with n-hexane—ethyl acetate—ethanol, 95:3:2, followed by derivatization with the anisaldehyde – sulfuric acid reagent. The UV/Vis/FLD detection was expanded by a biological and biochemical detection. Eight different effect-directed assays were performed including the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay as well as biochemical assays for inhibition of acetylcholinesterase, α- and β-glucosidases, α-amylase and tyrosinase as well as antimicrobial assays against Gram-negative Aliivibrio fischeri and Gram-positive Bacillus subtilis bacteria. The bioprofiling, subsequent direct analysis in real time mass spectrometry of the detected five bioactive compound zones and comparison with literature data resulted in the tentative assignment of caryophyllene oxide (hRF 20), α-humulene (hRF 26), carvacrol (hRF 40), methyl carvacrol ether (hRF 76) and caryophyllene (hRF 84). The antimicrobial activity against Gram-negative bacteria of PAEO was elicited by a mixture of the multi-potent compounds, whereas α-humulene was strongly acting against Gram-positive bacteria. The antioxidant effect was related to carvacrol and the inhibitory effect over AChE, tyrosinase and glucosidases to α-humulene. Graphical Abstract

中文翻译：

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通过平面色谱-效应导向分析结合实时高分辨率质谱中的直接分析对墨西哥 Plectranthus amboinicus (Lour.) 精油进行生物分析

摘要 在北墨西哥城收集了 Plectranthus amboinicus (Lour.) 的空中部分，以进行全面的效果导向分析。其水蒸馏的 P. amboinicus 精油 (PAEO) 在 HPTLC 硅胶板上用正己烷 - 乙酸乙酯 - 乙醇，95:3:2 进行分离，然后用茴香醛 - 硫酸试剂衍生化。UV/Vis/FLD 检测由生物和生化检测扩展。进行了八种不同的效应导向试验，包括 2,2-二苯基-1-苦基肼自由基清除试验以及抑制乙酰胆碱酯酶、α-和β-葡萄糖苷酶、α-淀粉酶和酪氨酸酶的生化试验以及针对革兰氏阴性 Aliivibrio fischeri 和革兰氏阳性枯草芽孢杆菌细菌。生物分析，随后对检测到的五个生物活性化合物区域进行实时质谱直接分析，并与文献数据进行比较，初步确定了石竹烯氧化物 (hRF 20)、α-葎草烯 (hRF 26)、香芹酚 (hRF 40)、甲基香芹酚醚(hRF 76) 和石竹烯 (hRF 84)。PAEO 对革兰氏阴性菌的抗菌活性是由多效化合物的混合物引起的，而 α-葎草烯对革兰氏阳性菌有很强的作用。抗氧化作用与香芹酚和乙酰胆碱酯酶、酪氨酸酶和葡萄糖苷酶对α-葎草烯的抑制作用有关。图形概要 香芹酚 (hRF 40)、甲基香芹酚醚 (hRF 76) 和石竹烯 (hRF 84)。PAEO 对革兰氏阴性菌的抗菌活性是由多效化合物的混合物引起的，而 α-葎草烯对革兰氏阳性菌有很强的作用。抗氧化作用与香芹酚和乙酰胆碱酯酶、酪氨酸酶和葡萄糖苷酶对α-葎草烯的抑制作用有关。图形概要 香芹酚 (hRF 40)、甲基香芹酚醚 (hRF 76) 和石竹烯 (hRF 84)。PAEO 对革兰氏阴性菌的抗菌活性是由多效化合物的混合物引起的，而 α-葎草烯对革兰氏阳性菌有很强的作用。抗氧化作用与香芹酚和乙酰胆碱酯酶、酪氨酸酶和葡萄糖苷酶对α-葎草烯的抑制作用有关。图形概要