Abstract Shankhpushpi, traded in the Indian market is having different controversial botanical sources, that is, Convolvulus pluricaulis Choisy (Convulvulaceae), Clitoria ternatea Linn. (Papilionaceae), and Evolvulus alsinoides Linn. (Convulvulaceae). Simple, accurate and precise RP-HPLC-PDA method was established for quality assessment and discrimination of Shankhpushpi samples using chromatographic profiling method. In distinction to the routine method of quality assessment which uses single or dual marker peaks, all chromatographic data (retention times/variables) were used. Fifteen shankhpushpi samples were purchased from the market including authenticated samples of all three C. pluricaulis, C. ternatea, E. alsinoides. A total of 18 samples were analyzed by HPLC and the dataset was then treated with multivariate analyses like PCA and HCA by using MINITAB software. Thus, the developed method was useful in discriminating the Shankhpushpi samples and for the perseverance of quality control. GRAPHICAL ABSTRACT

中文翻译：

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通过 HPLC 结合化学计量学方法对 Shankhpushpi 进行物种鉴别的指纹分析

摘要 在印度市场上交易的Shankhpushpi 具有不同的有争议的植物来源，即Convolvulus pluricaulis Choisy (Convul​​ulaceae)、Clitoria ternatea Linn。（凤蝶科）和 Evolvulus alsinoides Linn。（惊厥科）。建立了简单、准确和精密的 RP-HPLC-PDA 方法，用于使用色谱分析方法对 Shankhpushpi 样品进行质量评估和鉴别。与使用单或双标记峰的常规质量评估方法不同，使用了所有色谱数据（保留时间/变量）。从市场上购买了 15 个 shankhpushpi 样品，包括所有三种 C. pluricaulis、C. ternatea、E. alsinoides 的认证样品。通过 HPLC 分析了总共 18 个样品，然后使用 MINITAB 软件对数据集进行多变量分析，如 PCA 和 HCA。因此，开发的方法可用于区分 Shankhpushpi 样品和坚持质量控制。图形概要