ABSTRACT The oxidant, cholinesterase, and carbonic anhydrase inhibitions of essential oils and their major compounds of Leiotulus dasyanthus and molecular docking studies of α-pinene and δ-3-carene were evaluated in this study. Phenolic constituents and antioxidant activity of samples were determined by Folin-Ciocalteu’s, DPPH, and TBA processes. Cholinesterase inhibition effect was evaluated through Ellman’s method. GC-FID and GC/MS were used for analysing of essential oils. The root oil got the highest total phenolic content (898.87 mg/g). The utmost activity was shown flower oil (29.31 μg/mL) in the DPPH test. The root oil indicated considerable inhibition towards acetylcholinesterase (21.92%) and butyrylcholinesterase (91.33%) enzymes, respectively. α-pinene and δ-3-carene indicated high acetylcholinesterase inhibitory activity (66.44 and 48.71%, respectively). Cytosolic hCA I and II isoenzymes were influentially inhibited by flower and aerial part oils with 1.694 and 1.677 µM IC50 values, respectively. 1-Dodecanol (7.8%); β-pinene (63.3%), α-pinene (20.5%); octyl acetate (14.7%); hexyl octanoate (55.1%) were characterized as principle compounds of aerial parts, roots, flowers, and fruits respectively.

中文翻译：

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非生物碱乙酰胆碱酯酶和碳酸酐酶同工酶抑制剂的筛选 Leiotulus dasyanthus (K. Koch) Pimenov & Ostr. （伞形科）

摘要 本研究评估了大花精油及其主要化合物的氧化剂、胆碱酯酶和碳酸酐酶抑制作用，以及 α-蒎烯和 δ-3-蒎烯的分子对接研究。样品的酚类成分和抗氧化活性通过 Folin-Ciocalteu's、DPPH 和 TBA 方法测定。胆碱酯酶抑制作用通过Ellman法评价。GC-FID 和 GC/MS 用于分析精油。根油的总酚含量最高（898.87 mg/g）。在 DPPH 测试中，花油 (29.31 μg/mL) 的活性最高。根油分别对乙酰胆碱酯酶 (21.92%) 和丁酰胆碱酯酶 (91.33%) 具有显着的抑制作用。α-蒎烯和 δ-3-carene 显示出高乙酰胆碱酯酶抑制活性 (66.44 和 48. 71%，分别）。细胞溶质 hCA I 和 II 同工酶受到花和地上部分油的显着抑制，IC50 值分别为 1.694 和 1.677 µM。1-十二醇 (7.8%); β-蒎烯（63.3%）、α-蒎烯（20.5%）；乙酸辛酯（14.7%）；辛酸己酯 (55.1%) 分别被表征为地上部分、根、花和果实的主要化合物。