Abstract A new acylhydrazone (C12H12N4OS, HL) and its three transition metal complexes [M(HL)2](NO3)2 (M = Co, I; Cu, II; Zn, III) have been synthesized. The structures of above four compounds were determined by single-crystal X-ray diffraction analyses. HL belonged to orthorhombic system and Pbca space group. I and II belonged to triclinic system and P-1 space group, whereas III belonged to monoclinic system and P21/C space group. The thermal stabilities of four compounds were investigated by thermogravimetric analyses, and their maximum thermal decomposition peak temperatures were all more than 240 °C, showing high thermal stabilities. The binding behaviors of four compounds with CT-DNA were determined by UV–vis absorption spectra, viscosity and microcalorimetric experiments, all presenting intercalative modes. The interactions of four compounds with BSA were determined by fluorescence spectra and microcalorimetric experiments, showing static quenching effects, and the binding constants KA are (8.32 ± 0.36) × 103 M−1, (1.44 ± 0.40) × 105 M−1, (7.59 ± 0.09) × 104 M−1, and (4.68 ± 0.45) × 105 M−1 for HL, I, II, and III, respectively. Microcalorimetric experiments explored that the interactions of four compounds with CT-DNA or BSA were exothermic processes and the interaction times were all less than 43 min. Graphical Abstract

中文翻译：

---

新型酰基腙及其 Co(II)、Cu(II) 和 Zn(II) 配合物的合成、晶体结构、热稳定性、CT-DNA 和 BSA 结合特性

摘要 合成了一种新的酰腙（C12H12N4OS，HL）及其三种过渡金属配合物[M(HL)2](NO3)2（M = Co，I；Cu，II；Zn，III）。以上四种化合物的结构均通过单晶X射线衍射分析确定。HL属于正交系和Pbca空间群。I和II属于三斜晶系和P-1空间群，而III属于单斜晶系和P21/C空间群。通过热重分析研究了四种化合物的热稳定性，它们的最大热分解峰温度均超过 240 °C，显示出较高的热稳定性。四种化合物与 CT-DNA 的结合行为由紫外可见吸收光谱、粘度和微量热实验确定，所有这些都呈现嵌入模式。通过荧光光谱和微量热实验测定了四种化合物与BSA的相互作用，显示出静态猝灭效应，结合常数KA为（8.32±0.36）×103M-1，（1.44±0.40）×105M-1，（ HL、I、II 和 III 分别为 7.59 ± 0.09) × 104 M−1 和 (4.68 ± 0.45) × 105 M−1。微量热实验探索了四种化合物与CT-DNA或BSA的相互作用是放热过程，相互作用时间均小于43分钟。图形概要 微量热实验探索了四种化合物与CT-DNA或BSA的相互作用是放热过程，相互作用时间均小于43分钟。图形概要 微量热实验探索了四种化合物与CT-DNA或BSA的相互作用是放热过程，相互作用时间均小于43分钟。图形概要