ABSTRACT

Background: Dysfunction of the peripheral blood monocytes in the form of changes in their proportion, cytokines or pattern-recognition receptors (PRR) expressions may be involved in the pathogenesis of type 1 diabetes mellitus (T1DM). Our aim is to analyze the three monocyte subsets; classical, non-classical and intermediate monocytes and their expression of Toll-like receptors 2 (TLR-2) and 4 (TLR-4) in T1DM patients.

Methods: The peripheral blood monocytes of 20 T1DM patients were analyzed by Flow cytometry to measure their count and TLR-2 and TLR-4 expression.

Results: T1DM patients had more non-classical and intermediate monocytes, whereas classical monocytes were comparable between patients and control (20 healthy volunteers). Classical, non-classical and intermediate monocytes had no significant correlations with hemoglobin (Hb) A1C in controls, while all subsets showed positive correlations with HbA1C in T1DM. TLR-2 and TLR-4 expression were significantly increased in classical monocytes in patients, especially those with diabetic ketoacidosis (DKA), and both of them showed positive correlations with the duration of T1DM. The expression of TLR-2 inside non-classical monocytes showed a negative correlation with LDL cholesterol and TLR-4/TLR-2 ratio showed positive correlations with the duration of T1DM and negative correlations with total cholesterol. The expression of TLR-2 inside intermediate monocytes showed positive correlations with the duration of T1DM and TLR-4/TLR-2 ratio showed negative correlations with the duration of T1DM

Conclusions: The observed changes in both proportions and TLR-2 and TLR-4 expression of monocyte subsets can raise the possible role in the pathogenesis of early stages of T1DM and DKA.

Abbreviations APC: allophycocyanin; CBC: complete blood picture; DKA: diabetic acidosis; DM: diabetes mellitus; FITC: fluorescein isothiocyanate; FSC: forward scatter; Hb: haemoglobin; MFI: mean channel fluorescence intensity; PE: phycoerythrin; PRR: pattern-recognition receptors; SPSS: statistical package for the social sciences; SSC: side scatter; T1DM: Type1DM; TLRs: toll-like receptors

摘要

背景：外周血单核细胞的功能障碍以其比例、细胞因子或模式识别受体 (PRR) 表达的变化形式可能参与 1 型糖尿病 (T1DM) 的发病机制。我们的目标是分析三个单核细胞亚群；经典、非经典和中间单核细胞及其在 T1DM 患者中的 Toll 样受体 2 (TLR-2) 和 4 (TLR-4) 的表达。

方法：采用流式细胞术检测20例T1DM患者外周血单核细胞计数及TLR-2、TLR-4表达。

结果：T1DM 患者具有更多的非经典和中间单核细胞，而经典单核细胞在患者和对照（20 名健康志愿者）之间具有可比性。经典、非经典和中间单核细胞与对照组中的血红蛋白 (Hb) A1C 没有显着相关性，而所有亚组都与 T1DM 中的 HbA1C 呈正相关。TLR-2和TLR-4在患者经典单核细胞中的表达显着增加，尤其是糖尿病酮症酸中毒（DKA）患者，两者均与T1DM病程呈正相关。非经典单核细胞内TLR-2的表达与LDL胆固醇呈负相关，TLR-4/TLR-2比值与T1DM病程呈正相关，与总胆固醇呈负相关。

结论：观察到的单核细胞亚群比例和 TLR-2 和 TLR-4 表达的变化可以提高在 T1DM 和 DKA 早期发病机制中的可能作用。

缩写APC：别藻蓝蛋白；CBC：全血图片；DKA：糖尿病性酸中毒；DM：糖尿病；FITC：异硫氰酸荧光素；FSC：前向散射；Hb：血红蛋白；MFI：平均通道荧光强度；PE：藻红蛋白；PRR：模式识别受体；SPSS：社会科学统计软件包；SSC：侧向散射；T1DM：Type1DM；TLRs：toll样受体