An experiment was conducted to evaluate: (1) the effects of ensiling maize or sorghum grains after reconstitution on readily soluble fraction (a), potentially degradable fraction in the rumen (b) and rate constant for degradation of b (c) of dry matter (DM), organic matter (OM) and starch (STA); and (2) an appropriate incubation time for in situ or in vitro procedures to estimate in vivo digestibility. Four rumen-cannulated Nellore bulls (body weight = 262 ± 19.6 kg) distributed in a 4 × 4 Latin square were used. Diets were based on dry ground maize (DGM); or dry ground sorghum (DGS); or reconstituted ground maize silage; or reconstituted ground sorghum silage. In vitro and in situ incubations of the individual grains and diets were simultaneously performed with in vivo digestibility. In general, reconstituted grains and diets based on reconstituted grains presented greater (P < 0.05) fraction a and lower (P < 0.05) fraction b of DM, OM and STA compared to dry grains and diets based on dry grain. However, the magnitude of response of the reconstitution and ensiling process on DM and OM degradability parameter was greater for maize than that for sorghum. Moreover, no differences (P > 0.05) were observed between DGM- and DGS-based diets for c estimates. The results suggest that the reconstitution process promotes grains protein matrix breakdown increasing STA availability. The incubation times required for in vivo digestibility estimations of DM, OM and STA are 24 h for in situ and 36 h for in vitro procedures.

中文翻译：

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用于估计基于玉米或高粱的日粮降解参数和消化率的原位和体外技术

进行了一项实验来评估：（1）青贮玉米或高粱籽粒在复原后对易溶部分（一种)，瘤胃中可能降解的部分 (b) 和降解速率常数b(C) 的干物质 (DM)、有机物质 (OM) 和淀粉 (STA)；(2) 适当的孵育时间原位要么体外估计程序体内消化率。使用分布在 4 × 4 拉丁方格中的四只瘤胃插管 Nellore 公牛（体重 = 262 ± 19.6 kg）。日粮以干磨玉米（DGM）为基础；或干磨高粱 (DGS)；或重组的玉米青贮饲料；或重组的地面高粱青贮饲料。体外和原位单个谷物和日粮的孵化同时进行体内消化率。一般来说，再造谷物和基于再造谷物的日粮表现出更大的（磷< 0.05) 分数一种和更低（磷< 0.05) 分数bDM、OM 和 STA 与干粮和基于干粮的日粮相比。然而，玉米的重组和青贮过程对 DM 和 OM 降解参数的响应幅度大于高粱。此外，没有差异（磷> 0.05）在基于 DGM 和基于 DGS 的饮食之间观察到C估计。结果表明，重组过程促进谷物蛋白质基质分解，增加 STA 可用性。所需的孵育时间体内DM、OM 和 STA 的消化率估计为 24 小时原位和 36 小时体外程序。