ABSTRACT

We investigated the effects of acrylamide (AA) and vitamin E treatment during pregnancy on brain tissues of fetuses and on adult rats. Pregnant rats were divided into five groups: control, corn oil, vitamin E, AA, vitamin E +AA. The rats administered AA received10 mg/kg/day and those administered vitamin E received 100 mg/kg/day both by via oral gavage for 20 days. On day 20 of pregnancy, half of the pregnant rats were removed by cesarean section in each group. Morphological development parameters were measured in each fetus and histopathological, biochemical and genetic analyses were conducted on the fetuses. The remaining pregnant rats in each group gave birth to the fetuses vaginally and biochemical, histopathological, genetic and cognitive function tests were conducted when the pups were 8 weeks old. AA administration caused adverse effects on fetus number, fetal weight, crown-rump length, placenta and brain weight. AA negatively affected malondialdehyde, reduced glutathione, total oxidant and antioxidant status, brain derived neurotrophic factor (BDNF) levels, brain tissue morphology, histopathology error score and gene expression (BDNF/β‐actin mRNA ratio) in fetuses. AA administration caused disruption of biochemical, histopathological and cognitive functions in adult rats. Vitamin E provided protection against neurotoxicity in both fetuses and adult rats. We conclude that exposure to AA during pregnancy should be avoided and adequate amounts of antioxidants, such as vitamin E, should be consumed.

摘要

我们调查了怀孕期间丙烯酰胺（AA）和维生素E治疗对胎儿脑组织和成年大鼠的影响。妊娠大鼠分为五组：对照组，玉米油，维生素E，AA，维生素E + AA。给予AA的大鼠每天通过口服管饲20mg / kg /天，给予维生素E的大鼠100mg / kg /天，均为20天。在怀孕的第20天，通过剖宫产将每组中的一半怀孕的大鼠切除。测量每个胎儿的形态发育参数，并对胎儿进行组织病理学，生化和遗传分析。每组中其余的妊娠大鼠通过阴道分娩胎儿，并在幼犬8周大时进行生化，组织病理学，遗传和认知功能测试。AA给药对胎儿数量，胎儿体重，冠臀长，胎盘和脑重产生不利影响。AA对胎儿的丙二醛，谷胱甘肽降低，总氧化剂和抗氧化剂状态，脑源性神经营养因子（BDNF）水平，脑组织形态学，组织病理学错误评分和基因表达（BDNF /β-actinmRNA比）产生负面影响。AA给药导致成年大鼠的生化，组织病理学和认知功能受到破坏。维生素E可以保护胎儿和成年大鼠免受神经毒性。我们的结论是，应避免在怀孕期间接触AA，并应摄入足够量的抗氧化剂，例如维生素E。胎儿的总氧化剂和抗氧化剂状态，脑源性神经营养因子（BDNF）水平，脑组织形态，组织病理学错误评分和基因表达（BDNF /β-actinmRNA比）。AA给药导致成年大鼠的生化，组织病理学和认知功能受到破坏。维生素E可以保护胎儿和成年大鼠免受神经毒性。我们的结论是，应避免在怀孕期间接触AA，并应摄入足够量的抗氧化剂，例如维生素E。胎儿的总氧化剂和抗氧化剂状态，脑源性神经营养因子（BDNF）水平，脑组织形态，组织病理学错误评分和基因表达（BDNF /β-actinmRNA比）。AA给药导致成年大鼠的生化，组织病理学和认知功能受到破坏。维生素E可以保护胎儿和成年大鼠免受神经毒性。我们的结论是，应避免在怀孕期间接触AA，并应摄入足够量的抗氧化剂，例如维生素E。维生素E可以保护胎儿和成年大鼠免受神经毒性。我们的结论是，应避免在怀孕期间接触AA，并应摄入足够量的抗氧化剂，例如维生素E。维生素E可以保护胎儿和成年大鼠免受神经毒性。我们的结论是，应避免在怀孕期间接触AA，并应摄入足够量的抗氧化剂，例如维生素E。