ABSTRACT

Periodontitis is a common chronic infection of dental tissues. Ozone therapy (OT) and low level laser therapy (LLLT) are useful treatments for periodontitis. We investigated the effects of OT and LLLT on periodontal disease-induced bone destruction in rats with experimentally induced periodontitis (EP). We used 30 male Wistar rats divided into three groups: control, OT and LLLT. EP was induced by placing a 3.0 silk suture around the cervix of the left mandibular first molar tooth. OT was performed using an ozone generator at 80% concentration. LLLT was applied using a diode laser. Both OT and LLLT were performed for two weeks at two day intervals. Histomorphometric and immunohistochemical analyses also were performed. Alveolar bone loss was significantly less in the LLLT group compared to the control group. The number of HIF-1α positive cells was significantly less in the LLLT group compared to the control group. We found significantly fewer RANKL-positive cells in the OT group compared to the control group. The number of osteoprotegerin (OPG) positive cells was significantly greater for the LLLT group than for the control group. Although both treatments produced positive effects, LLLT appears to be more effective for increasing alveolar bone formation.

摘要

牙周炎是牙齿组织的常见慢性感染。臭氧疗法（OT）和低剂量激光疗法（LLLT）是治疗牙周炎的有用方法。我们调查了OT和LLLT对实验性牙周炎（EP）大鼠牙周疾病引起的骨破坏的影响。我们使用了30只雄性Wistar大鼠，分为三组：对照组，OT和LLLT。通过在左下颌第一磨牙的子宫颈周围放置3.0丝缝线来诱发EP。使用浓度为80％的臭氧发生器进行OT。使用二极管激光器施加LLLT。OT和LLLT均间隔两天进行两周。还进行了组织形态计量学和免疫组化分析。与对照组相比，LLLT组的牙槽骨丢失明显更少。与对照组相比，LLLT组中HIF-1α阳性细胞的数量明显减少。我们发现与对照组相比，OT组的RANKL阳性细胞明显减少。LLLT组的骨保护素（OPG）阳性细胞数量明显多于对照组。尽管两种治疗均产生积极效果，但LLLT似乎在增加牙槽骨形成方面更有效。