ABSTRACT

We investigated the expression of A disintegrin and metalloprotease with thrombospondin type I repeats-13 (ADAMTS-13) in the central nervous system (CNS), because it is related to blood-brain barrier (BBB) permeability. We also investigated 8-OHdG, caspase-3 and neuronal nitric oxide synthase (nNOS) expression for the cytotoxic effects of oxidative stress (OS) and nNOS, and their relation to apoptosis. We also investigated the neuroimmunopathology caused by L. monocytogenes. Brain tissues were obtained from 10 lambs and 10 kids with listeric meningoencephalitis, and healthy brain tissue from six lambs and six kids. Serial sections of brain were stained by hematoxylin and eosin (H & E), and using immunohistochemistry (IHC) for L. monocytogenes antigen, ADAMTS-13, 8-hydroxy-2′-deoxyguanosine (8-OHdG), nNOS and caspase-3. We found that ADAMTS-13, 8-OHdG, nNOS and caspase-3 expression in the brain was increased in L. Monocytogenes infected animals compared to uninfected controls. Intense staining for 8-OHdG was observed only in neurons and glia that were exposed to OS. ADAMTS-13 was increased significantly, which may play a role in regulating and protecting BBB integrity and cells of the CNS in cases of listeric encephalitis. Increased expression of ADAMTS-13 may be critical for supporting the survival of neurons and glia. We found that L. monocytogenes-related increases in OS and nNOS, and that the associated apoptosis, may participate in neurodegeneration and neuropathology in listeric encephalitis.

摘要

我们调查了中枢神经系统（CNS）中血小板反应蛋白I型重复序列13（ADAMTS-13）的A整合素和金属蛋白酶的表达，因为它与血脑屏障（BBB）的通透性有关。我们还调查了8-OHdG，caspase-3和神经元一氧化氮合酶（nNOS）的表达对氧化应激（OS）和nNOS的细胞毒性作用及其与凋亡的关系。我们还调查了由单核细胞增生李斯特菌引起的神经免疫病理学。从10只羊羔和10名患利斯特性脑膜脑炎的孩子那里获取脑组织，从6只羊羔和6名孩子中获取健康的大脑组织。用苏木精和曙红（H＆E）染色大脑的连续切片，并用免疫组织化学（IHC）染色单核细胞增生李斯特菌抗原，ADAMTS-13、8-羟基-2'-脱氧鸟苷（8-OHdG），nNOS和caspase-3。我们发现，与未感染对照相比，L。Monocytogenes感染动物的大脑中ADAMTS-13、8-OHdG，nNOS和caspase-3表达增加。仅在暴露于OS的神经元和神经胶质中观察到8-OHdG的强烈染色。ADAMTS-13显着增加，在李斯特脑炎病例中可能在调节和保护BBB完整性和CNS细胞中起作用。ADAMTS-13表达的增加对于支持神经元和神经胶质的存活可能至关重要。我们发现单核细胞增生李斯特氏菌相关的OS和nNOS升高，以及相关的细胞凋亡可能参与了李斯特脑炎的神经退行性变和神经病理学。