Failure of apoptosis contributes to leukemia progression. We investigated extracts of a native Iranian plant, Satureja bachtiarica, for possible anti-leukemia activity by induction of apoptosis and changes to the cell cycle. Growth inhibition caused by aqueous, butanol, dichloromethane and hexane extracts of S. bachtiarica on K562 and Jurkat leukemia cells was assessed using a colorimetric assay. Extracts were analyzed for induction of apoptosis and cell cycle arrest using flow cytometry and measurement of caspase-3 activity. Dichloromethane and hexane extracts inhibited leukemia cell proliferation in a dose-dependent manner. The IC₅₀ values of these extracts were 22−33 µg/ml. Flow cytometric determination of annexinV/propidium iodide positive cells verified a significantly increased percentage of apoptotic cells compared to negative controls. Both 50 μg/ml dichloromethane and hexane extracts induced apoptosis in 89–97% of K562 and 94–97% of Jurkat cells 48 h after treatment. The effects of extracts on the cell cycle included significantly increased numbers of K562 and Jurkat cells in the subG1 phase and decreased numbers of cells in the G1, S and G2/M phases. After 24 h, we found increased levels of caspase-3 activation in cells treated with 25 μg/ml dichloromethane and hexane extracts compared to untreated cells. Our findings indicate the anti-leukemic effects of dichloromethane and hexane extracts of S. bachtiarica due to induction of apoptosis and inhibition of cell cycle progression.

细胞凋亡的失败有助于白血病的进展。我们调查了伊朗本地植物Satureja bachtiarica的提取物，通过诱导凋亡和改变细胞周期来可能具有抗白血病活性。使用比色测定法评估了S. bachtiarica的丁醇，二氯甲烷和己烷提取液对K562和Jurkat白血病细胞的生长抑制作用。使用流式细胞仪分析了提取物的凋亡诱导作用和细胞周期阻滞，并测定了caspase-3活性。二氯甲烷和己烷提取物以剂量依赖的方式抑制白血病细胞的增殖。IC ₅₀这些提取物的值为22-33 µg / ml。AnnexinV /碘化丙啶阳性细胞的流式细胞仪测定证实，与阴性对照相比，凋亡细胞的百分比显着增加。处理后48小时，50μg/ ml的二氯甲烷和己烷提取物均可诱导89–97％的K562细胞和94–97％的Jurkat细胞凋亡。提取物对细胞周期的影响包括subG1期的K562和Jurkat细胞数量明显增加，而G1，S和G2 / M期的细胞数量减少。24小时后，我们发现与未处理的细胞相比，用25μg/ ml二氯甲烷和己烷提取物处理的细胞中caspase-3激活水平增加。我们的发现表明，巴氏链球菌的二氯甲烷和己烷提取物具有抗白血病作用 由于诱导凋亡和抑制细胞周期进程。