Abstract Silver nanoparticles (AgNPs) have been wildly used in agriculture, cosmetics, medicine, and food conservation fields due to their excellent antibacterial activity. The growing use of AgNPs products results that they can be inevitably released into the environment. Additionally, silver (Ag+) can easily find its way to the environmental waters from AgNPs. To gain deep insight into the transformation between AgNPs and Ag+ in the organisms or environment, and evaluate the toxicity of AgNPs and Ag+, various speciation analysis methods are developed. This paper presents a brief and systemic review for the speciation analysis of AgNPs and Ag+ based on the recently developed separation techniques coupled with atomic spectroscopy, including extraction method, chromatographic method, cation exchange reaction (CER) and single particles (SP) detection. Future perspectives in this field are also discussed.

中文翻译：

---

基于原子光谱法的银纳米粒子和银离子形态分析方法的新进展

摘要 银纳米粒子（AgNPs）因其优异的抗菌活性而被广泛应用于农业、化妆品、医药和食品保护领域。越来越多地使用 AgNPs 产品导致它们不可避免地被释放到环境中。此外，银 (Ag+) 可以很容易地从 AgNPs 找到进入环境水域的途径。为了深入了解生物体或环境中 AgNPs 和 Ag+ 之间的转化，并评估 AgNPs 和 Ag+ 的毒性，开发了各种形态分析方法。本文基于最近开发的分离技术结合原子光谱，包括萃取法、色谱法、阳离子交换反应 (CER) 和单粒子 (SP) 检测。还讨论了该领域的未来前景。