Abstract A new automated system based on a multi-pumping flow system (MPFS) for the spectrophotometric determination of total iron in various wine samples is presented. This analytical protocol is based on the complex formation between Fe (II) and 2-(5-bromo-2-pyridylazo)-5-(diethylamino)-phenol (Br-PADAP). The addition of ascorbic acid allows the determination of total iron by reducing the Fe (III) present in the samples to Fe (II). Several physical and chemical parameters, including the mixing coil length, the sample volume, the chromogenic reagent concentration, the pH, and the ascorbic acid concentration have been characterized in order to optimize the analytical conditions. The limits of detection and quantification were 34 µg L−1 and 114 µg L−1 respectively. The developed procedure requires minimal sample preparation.

中文翻译：

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用于测定葡萄酒中总铁的自动分光光度法多泵流量系统

摘要 提出了一种基于多泵流系统 (MPFS) 的新型自动化系统，用于分光光度法测定各种葡萄酒样品中的总铁。该分析方案基于 Fe (II) 和 2-(5-bromo-2-pyridylazo)-5-(diethylamino)-phenol (Br-PADAP) 之间的复合物形成。添加抗坏血酸可以通过将样品中存在的 Fe (III) 还原为 Fe (II) 来测定总铁。一些物理和化学参数，包括混合线圈长度、样品体积、显色试剂浓度、pH 值和抗坏血酸浓度已被表征，以优化分析条件。检测限和定量限分别为 34 µg L-1 和 114 µg L-1。开发的程序需要最少的样品制备。