Abstract Amino-MIL-53(Al) nanosheets were synthesized by a hydrothermal reaction and were employed as a fluorescent probe because of their high quantum yield and excellent stability. The amino-MIL-53(Al) nanosheets have a maximum fluorescence emission peak at 435 nm using an excitation wavelength of 335 nm. The fluorescent intensity of luminophor at 435 nm was linearly quenched when mercury (II) (Hg2+) was added, which occurred due to the adsorption of Hg2+ onto the luminophor surface. Under these conditions, the fluorescent signal of the system is in the “turn-off” state. Following the addition of glutathione (GSH) into the system, the quenched fluorescence intensity may be partially restored based on the reaction between glutathione and Hg2+. In this condition, the fluorescent signal of the amino-MIL-53(Al)-Hg2+ system is in the “turn-on” state. Therefore, the amino-MIL-53(Al) nanosheets were successfully used as a “turn-off-on” fluorescent sensing system to determine the concentrations of Hg2+ in Tibetan medicine and glutathione in rabbit serum. A good correlation coefficient was obtained between the quenching of the fluorescence intensity of luminophor and the concentration of Hg2+ using the optimized conditions. A linear range was acquired from 1.96 nM to 38.27 μM with a detection limit of 0.23 nM of Hg2+ based upon a signal-to-noise ratio of three. For glutathione, the linear dynamic range was from 210 nM to 15.25 μM with a detection limit equal to 8.11 nM based upon a signal-to-noise ratio of three. This developed sensor system has the advantages of high selectivity, favorable sensitivity and simple operation for the determination of Hg2+ and glutathione in real samples.

中文翻译：

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使用氨基-MIL-53(Al) 纳米片荧光测定汞 (II) 和谷胱甘肽

摘要 Amino-MIL-53(Al) 纳米片是通过水热反应合成的，并因其高量子产率和优异的稳定性而被用作荧光探针。氨基-MIL-53(Al) 纳米片在 435 nm 处具有最大荧光发射峰，激发波长为 335 nm。当添加汞 (II) (Hg2+) 时，发光体在 435 nm 处的荧光强度线性猝灭，这是由于 Hg2+ 吸附到发光体表面而发生的。在这些条件下，系统的荧光信号处于“关闭”状态。在系统中加入谷胱甘肽 (GSH) 后，基于谷胱甘肽与 Hg2+ 之间的反应，淬灭的荧光强度可能会部分恢复。在这种情况下，氨基-MIL-53(Al)-Hg2+ 系统的荧光信号处于“开启”状态。因此，氨基-MIL-53(Al)纳米片成功地用作“关闭-开启”荧光传感系统来测定藏药中Hg2+和兔血清中谷胱甘肽的浓度。优化条件下发光体荧光强度的猝灭与Hg2+浓度之间具有良好的相关系数。基于 3 的信噪比，获得了从 1.96 nM 到 38.27 μM 的线性范围，Hg2+ 的检测限为 0.23 nM。对于谷胱甘肽，线性动态范围为 210 nM 至 15.25 μM，基于信噪比为 3 的检测限等于 8.11 nM。该传感器系统具有选择性高、灵敏度好、操作简单等优点，适用于实际样品中Hg2+和谷胱甘肽的测定。氨基-MIL-53(Al) 纳米片成功用作“关闭-开启”荧光传感系统，用于测定藏药中 Hg2+ 和兔血清中谷胱甘肽的浓度。优化条件下发光体荧光强度的猝灭与Hg2+浓度之间具有良好的相关系数。基于 3 的信噪比，获得了从 1.96 nM 到 38.27 μM 的线性范围，Hg2+ 的检测限为 0.23 nM。对于谷胱甘肽，线性动态范围为 210 nM 至 15.25 μM，基于信噪比为 3 的检测限等于 8.11 nM。该传感器系统具有选择性高、灵敏度好、操作简单等优点，适用于实际样品中Hg2+和谷胱甘肽的测定。氨基-MIL-53(Al) 纳米片成功用作“关闭-开启”荧光传感系统，用于测定藏药中 Hg2+ 和兔血清中谷胱甘肽的浓度。优化条件下发光体荧光强度的猝灭与Hg2+浓度之间具有良好的相关系数。基于 3 的信噪比，获得了从 1.96 nM 到 38.27 μM 的线性范围，Hg2+ 的检测限为 0.23 nM。对于谷胱甘肽，线性动态范围为 210 nM 至 15.25 μM，基于信噪比为 3 的检测限等于 8.11 nM。该传感器系统具有选择性高、灵敏度好、操作简单等优点，适用于实际样品中Hg2+和谷胱甘肽的测定。