ABSTRACT

Formation of biofilms allows bacterial cells to survive in adverse environments. Nontuberculous mycobacteria are ubiquitous in aqueous environments, where they adhere to surfaces and create a biofilm. This has led to the emergence of healthcare-associated infections and the use of biomaterials in medicine. Essential oils (EO) are substances of natural origin whose effect on microorganisms has been the subject of numerous studies. Here, we investigated the effect of Juniperus communis EO on nontuberculous mycobacteria and their early and mature biofilm formation in sterilised tap water. The combination of Juniperus communis EO and increasing ambient temperature showed a synergistic effect on the reduction of biofilm formation of Mycobacterium avium and Mycobacterium intracellulare on a polystyrene surface. A significant antibiofilm effect of Juniperus communis EO was also found at subinhibitory concentrations, suggesting a potential role for it as an alternative disinfectant of natural water.

摘要

生物膜的形成使细菌细胞能够在不利的环境中生存。非结核分枝杆菌在水环境中无处不在，它们附着在表面并形成生物膜。这导致了医疗保健相关感染的出现以及生物材料在医学中的使用。精油 (EO) 是天然来源的物质，其对微生物的影响已成为众多研究的主题。在这里，我们研究了杜松EO 对非结核分枝杆菌的影响及其在无菌自来水中早期和成熟的生物膜形成。杜松EO 和提高环境温度的组合对减少鸟分枝杆菌的生物膜形成和聚苯乙烯表面上的胞内分枝杆菌。在亚抑制浓度下还发现杜松EO具有显着的抗生物膜作用，这表明它作为天然水的替代消毒剂具有潜在作用。