Research on cryptic species complexes has reached a consensus on the necessity of integrating multiple sources of evidence. Reduced representation library techniques such as Genotyping-by-Sequencing (GBS) have proven useful to study these groups. Both integrative taxonomy and genome-wide single nucleotide polymorphism (SNP) data remain to be widely applied to earthworms, an animal group with widespread presence of cryptic diversity. The genus Carpetania (formerly the Hormogaster elisae species complex) was found to contain six deeply divergent genetic lineages and some inconspicuous morphological differentiation based on a handful of Sanger-sequenced markers. Marchán et al. (submitted) delimited three well-supported species-level clades on the basis of a genome-wide SNP dataset and geometric morphometric analyses, highlighting the necessity of a formal taxonomic description of these taxa. In this work, further analyses are applied to the SNP data and a thorough morphological study is performed in order to provide an integrative description of two new species and to redescribe Carpetania elisae. Species-specific SNPs are identified and used as diagnostic characters, and genome-wide and cytochrome oxidase C subunit 1 (COI) genetic distances are compared finding a strong correlation between them. The taxonomic description of these three cryptic species provides a useful tool to include them effectively in ecological studies and biodiversity conservation actions. www.zoobank.org/urn:lsid:zoobank.org:act:19CC41D7-BD25-4337-BED5-CDB04CAFB537 www.zoobank.org/urn:lsid:zoobank.org:act:206EE090-4AF5-4D12-82CA-F4A6494E90D4 www.zoobank.org/urn:lsid:zoobank.org:pub:2797FDDF-EA3D-4347-96C6-E632DC26CDA3

中文翻译：

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蚯蚓属 Carpetania (Oligochaeta, Hormogastridae) 中三个隐匿物种的基因组综合分类学描述

对隐性物种复合体的研究已经就整合多种证据来源的必要性达成了共识。简化表示库技术，如基因分型测序 (GBS) 已被证明对研究这些群体很有用。综合分类法和全基因组单核苷酸多态性 (SNP) 数据仍有待广泛应用于蚯蚓，蚯蚓是一种广泛存在的隐秘多样性的动物群体。发现 Carpetania 属（以前称为 Hormogaster elisae 物种复合体）包含六个截然不同的遗传谱系和一些基于少数 Sanger 测序标记的不显眼的形态分化。马尔坎等人。（提交）基于全基因组 SNP 数据集和几何形态测量分析，划定了三个得到充分支持的物种级进化枝，强调对这些分类群进行正式分类描述的必要性。在这项工作中，对 SNP 数据进行了进一步分析，并进行了彻底的形态学研究，以提供两个新物种的综合描述并重新描述 Carpetania elisae。物种特异性 SNP 被鉴定并用作诊断特征，并且比较全基因组和细胞色素氧化酶 C 亚基 1 (COI) 的遗传距离，发现它们之间存在很强的相关性。这三个神秘物种的分类学描述提供了一个有用的工具，可以将它们有效地纳入生态研究和生物多样性保护行动中。www.zoobank.org/urn:lsid:zoobank.org:act:19CC41D7-BD25-4337-BED5-CDB04CAFB537 www.zoobank.org/urn:lsid:zoobank.org:act:206EE090-4AF5-4D12-864CA-F4A4E4A www.zoobank.org/urn:lsid:zoobank.org:pub: