Impairment of antioxidant enzymes activities has been well reported in several human diseases. Effective anti-aging strategies involving antioxidant supplementation and/or caloric restriction (CR) are receiving a great attention to mitigate free radical mediated oxidative damage in several disease conditions to improve active longevity. Therefore, in this work, we have evaluated the protective effect of quercetin under non restriction (NR) and caloric restriction (CR) conditions on the sensitivity of Saccharomyces cerevisiae mutant strains (sod1∆, sod2∆, cat1∆, ctt1∆, tsa1∆, and glr11∆) deficient in antioxidant defense systems (superoxide dismutases, catalases, glutathione reductase, thioredoxin peroxidase) against H2 O2 induced oxidative stress. Our results demonstrate that quercetin in combination with CR has strongly reduced the H2 O2 mediated stress in the yeast mutant cells compared to NR conditions. Further, we show that quercetin in combination with CR enhanced the percentage viability of yeast cells during chronological aging. Our research findings suggest that antioxidant supplementation in combination with CR might have potent beneficial effects than individual therapies against free radical mediated oxidative stress.

中文翻译：

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槲皮素联合热量限制对氧化应激诱导的酿酒酵母细胞死亡的保护作用

抗氧化酶活性受损已在几种人类疾病中得到充分报道。包括抗氧化剂补充剂和/或热量限制 (CR) 在内的有效抗衰老策略正在受到极大关注，以减轻几种疾病条件下自由基介导的氧化损伤，以延长活跃寿命。因此，在这项工作中，我们评估了槲皮素在非限制性 (NR) 和热量限制 (CR) 条件下对酿酒酵母突变菌株 (sod1∆, sod2∆, cat1∆, ctt1∆, tsa1∆) 敏感性的保护作用, 和 glr11∆) 缺乏抗氧化防御系统（超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶、硫氧还蛋白过氧化物酶）来对抗 H2 O2 诱导的氧化应激。我们的结果表明，与 NR 条件相比，槲皮素与 CR 的组合大大降低了酵母突变细胞中 H2O2 介导的压力。此外，我们表明槲皮素与 CR 结合提高了酵母细胞在时间老化过程中的存活率。我们的研究结果表明，抗氧化剂补充剂与 CR 相结合可能比针对自由基介导的氧化应激的单独疗法具有更有效的有益效果。