The article presents the results of a study on obtaining mutant doubled haploids of turnip rape. The culture medium for producing embryos in isolated microspore culture was optimized. The optimal medium formula was NLN + 13% S + 0.05 mg/L NAA + 0.05 mg/L BA. Embryos derived from isolated microspore culture were treated with EMS mutagen in three concentrations (4 mM, 8 mM, and 12 mM). Mutant doubled haploid plants and their seeds were obtained. Agronomic evaluation and analysis of fatty acid composition in mutant lines seeds showed that they had valuable traits in terms of seed oil quality and yield. Analysis of the fatty acid composition of the seeds of the obtained mutant doubled haploids showed an increase in the percentage of oleic acid (~11%–12%) in comparison with donor cultivars. Our results showed that mutagenesis of embryos from a culture of isolated microspores has potential for improving the qualitative traits of turnip rape.

中文翻译：

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萝卜油菜小孢子胚的诱变处理以增加油酸含量

本文介绍了获得萝卜油菜突变双倍单倍体的研究结果。优化了用于在分离的小孢子培养物中产生胚胎的培养基。最佳培养基配方为NLN + 13％S + 0.05 mg / L NAA + 0.05 mg / L BA。使用三种浓度（4 mM，8 mM和12 mM）的EMS诱变剂处理源自分离的小孢子培养物的胚胎。获得了突变的双倍单倍体植物及其种子。对突变株系种子中脂肪酸组成的农艺学评价和分析表明，它们在种子油品质和产量方面具有有价值的性状。对获得的突变单倍体单倍体种子的脂肪酸组成的分析表明，与供体品种相比，油酸百分比有所增加（〜11％–12％）。