An interspecific peanut hybrid population was generated from a cross between Arachis diogoi, a wild species with resistance to spotted wilt, and “Gregory”, a susceptible cultivar. The objectives of the study are to identify successful introgression of A. diogoi's genome and to evaluate the spotted wilt resistance in the introgression population. Sixty‐three putative introgression lines were genotyped using 137 polymorphic simple sequence repeat markers, out of which 14 markers located on seven of the 20 peanut chromosomes showed introgression of A. diogoi genome into at least one of the introgression lines. In addition, genotyping by sequencing of a subset of the population revealed SNP loci displaying introgression of A. diogoi genome on every chromosome of the A sub‐genome of the introgression lines, with the most introgressed loci on chromosome A05. Several introgression lines showed consistent spotted wilt resistance, suggesting successful genomic introgression of A. diogoi contributing to the resistance.

中文翻译：

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鉴定野生种花生到种间花生杂种的染色体渗入

种间花生杂种种群是由对斑枯病具有抗性的野生物种花生和易感品种“格雷戈里”之间的杂交产生的。该研究的目的是鉴定成功地渗入A. diogoi基因组并评估渗入种群中发现的抗枯萎病。使用137个多态性简单序列重复标记对63个推定的基因渗入系进行了基因分型，其中位于20个花生染色体中的7个中的14个标记显示了A. diogoi基因组渗入了至少一个渗入系。此外，通过对一部分人口进行测序进行基因分型，发现SNP基因座显示出渗入A. diogoi基因渗入系A亚基因组每个染色体上的基因组，基因座A05在基因渗入最多。几个渗入系显示出一致的斑点枯萎抗性，表明成功地对A. diogoi进行了基因组渗入。