BACKGROUND Rheumatoid arthritis (RA) is an autoimmune disease that leads to small joints irreversible destruction. Despite intense efforts, the pathophysiology of RA currently remains unclear. We aimed to gain insight into the pathophysiology process in peptidomic perspective and to identify bioactive peptides for RA treatment. METHODS The endogenous peptides in synovial tissue between control and rheumatoid arthritis group were identified by liquid chromatography-mass spectrometry (LC-MS/MS). Since the biological function of peptides were always associated with precursor proteins, the potential function of the differentially peptides were predicted by GO and pathway analysis of their precursors. Besides, peptides located in the domains of their precursors were identified. Finally, we determined the impact of galectin-1 derived peptide by administration on the damage to MH7A cells caused by TNF-α. RESULTS Totally, 141 down-regulated peptides and 10 up-regulated peptides were identified (Fold change > 1.5 and P < 0.05). It indicated that these differentially peptides were tightly involved in the pathophysiology process of RA preliminarily. Finally, we identified a peptide derived from the domain of galectin-1 could inhibit the abnormal proliferation induced by TNF-α and promoted apoptosis of MH7A. CONCLUSION In summary, our study provided a better understanding of endogenous peptides in RA. We found a peptide that might be used in anti-RA treatment.

中文翻译：

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滑膜组织的肽组学分析揭示了半乳糖凝集素 1 衍生肽是一种潜在的抗类风湿性关节炎生物活性分子

背景类风湿性关节炎(RA)是导致小关节不可逆破坏的自身免疫性疾病。尽管付出了巨大的努力，但 RA 的病理生理学目前仍不清楚。我们旨在从肽组学的角度深入了解病理生理学过程，并确定用于 RA 治疗的生物活性肽。方法采用液相色谱-质谱法(LC-MS/MS)鉴定对照组和类风湿关节炎组滑膜组织中的内源性多肽。由于肽的生物学功能总是与前体蛋白相关，因此差异肽的潜在功能可以通过GO及其前体的通路分析来预测。此外，还鉴定了位于其前体结构域中的肽。最后，我们通过给药确定了半乳糖凝集素 1 衍生肽对 TNF-α 对 MH7A 细胞损伤的影响。结果共鉴定出141个下调肽段和10个上调肽段（倍数变化> 1.5且P < 0.05）。初步表明这些差异肽与RA的病理生理过程密切相关。最后，我们确定了一种来源于半乳糖凝集素-1 结构域的肽可以抑制 TNF-α 诱导的异常增殖并促进 MH7A 的细胞凋亡。结论 总之，我们的研究提供了对 RA 中内源性肽的更好理解。我们发现了一种可用于抗 RA 治疗的肽。0.05）。初步表明这些差异肽与RA的病理生理过程密切相关。最后，我们确定了一种来源于半乳糖凝集素-1 结构域的肽可以抑制 TNF-α 诱导的异常增殖并促进 MH7A 的细胞凋亡。结论 总之，我们的研究提供了对 RA 中内源性肽的更好理解。我们发现了一种可用于抗 RA 治疗的肽。0.05）。初步表明这些差异肽与RA的病理生理过程密切相关。最后，我们确定了一种来源于半乳糖凝集素-1 结构域的肽可以抑制 TNF-α 诱导的异常增殖并促进 MH7A 的细胞凋亡。结论 总之，我们的研究提供了对 RA 中内源性肽的更好理解。我们发现了一种可能用于抗 RA 治疗的肽。