This study aims to explore the key and differentially expressed long non-coding RNAs (lncRNAs) and elucidates their possible mechanisms in malignant-transformed Human bronchial epithelial (BEAS-2B) cells induced by coal tar pitch extracts (CTPE). BEAS-2B cells were stimulated with 2.4 μg/ml CTPE, then passaged for three times which were named CTPE1 and then passaged until passage 30 (CTPE30). The results showed that cells of CTPE30 appeared abnormal morphology. Furthermore, migration, clonality and proliferation of cells in CTPE group were significantly increased compared with those in control groups. However, the apoptosis of cells in CTPE group was inhibited. A total of 569 differentially expressed mRNAs and 707 differentially expressed lncRNAs were screened out, among which four lncRNAs were validated and were consistent with the microarray results. 32 target genes were screened out by Co-expression network. The study suggests that differentially expressed lncRNAs may play a potential role in lung carcinogenesis

这项研究旨在探讨关键和差异表达的长非编码RNA（lncRNA），并阐明其在煤焦油沥青提取物（CTPE）诱导的恶性转化人支气管上皮细胞（BEAS-2B）中的可能机制。用2.4μg/ ml CTPE刺激BEAS-2B细胞，然后传代3次，称为CTPE1，然后传代至第30代（CTPE30）。结果显示CTPE30的细胞出现异常形态。此外，与对照组相比，CTPE组的细胞迁移，克隆性和增殖显着增加。但是，CTPE组的细胞凋亡受到抑制。总共筛选出569个差异表达的mRNA和707个差异表达的lncRNA，其中四个lncRNA已验证，与微阵列结果一致。通过共表达网络筛选出32个靶基因。研究表明，差异表达的lncRNA可能在肺癌的发生中起潜在作用。