NAC (NAM, ATAF1/2, and CUC2) transcription factors play important roles in the process of abiotic stress response in plants. However, little is known about the functional roles of NACs in Picea (P.) wilsonii. In this study, we functionally characterized a novel P. wilsonii NAC transcription factor PwNAC30 by heterologous expression in Arabidopsis. The results showed that PwNAC30 is mainly localized in the nucleus by transient expression in Nicotiana benthamiana and can function as a nuclear localization transcription factor. β-Glucuronidase (GUS) staining in transgenic Arabidopsis (PwNAC30-promoter-GUS) confirmed the expression pattern of PwNAC30 throughout the plant development process, which is expressed in most of the plant tissues except stamens and petals. Overexpression of PwNAC30 in Arabidopsis significantly repressed the tolerance of seedlings and mature plants to both drought and salt stresses, while exerting no influence on growth and development of plants. The accumulation of reactive oxygen species (ROS) was significantly increased in transgenic plants, and the expression of some stress-responsive genes was obviously inhibited in PwNAC30 overexpression lines. Our study reveals that PwNAC30 functions as a negative regulator of plant tolerance to drought or salt stress, which provides new insights into abiotic tolerance mechanisms in woody plants.

中文翻译：

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Picea wilsonii NAC 转录因子 PwNAC30 负调控转基因拟南芥的非生物胁迫耐受性

NAC（NAM、ATAF1/2 和 CUC2）转录因子在植物非生物胁迫响应过程中发挥重要作用。然而，关于 NAC 在云杉 (P.) wilsonii 中的功能作用知之甚少。在这项研究中，我们通过在拟南芥中的异源表达功能表征了一种新型 P. wilsonii NAC 转录因子 PwNAC30。结果表明，PwNAC30主要通过在本氏烟草中的瞬时表达定位于细胞核中，并且可以作为细胞核定位转录因子发挥作用。转基因拟南芥（PwNAC30-启动子-GUS）中的β-葡萄糖醛酸酶（GUS）染色证实了PwNAC30在整个植物发育过程中的表达模式，其在除雄蕊和花瓣外的大多数植物组织中都有表达。PwNAC30 在拟南芥中的过表达显着抑制了幼苗和成熟植物对干旱和盐胁迫的耐受性，而对植物的生长发育没有影响。转基因植物中活性氧（ROS）的积累显着增加，PwNAC30过表达株系中一些胁迫响应基因的表达明显受到抑制。我们的研究表明，PwNAC30 作为植物对干旱或盐胁迫耐受性的负调节因子，为木本植物的非生物耐受机制提供了新的见解。PwNAC30过表达株系中一些胁迫响应基因的表达明显受到抑制。我们的研究表明，PwNAC30 作为植物对干旱或盐胁迫耐受性的负调节因子，为木本植物的非生物耐受机制提供了新的见解。PwNAC30过表达株系中一些胁迫响应基因的表达明显受到抑制。我们的研究表明，PwNAC30 作为植物对干旱或盐胁迫耐受性的负调节因子，为木本植物的非生物耐受机制提供了新的见解。