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Loss of viability and vigour in the course of short-term storage of Pityrocarpa moniliformis seeds cannot be attributed to reserve degradation
New forests ( IF 2.2 ) Pub Date : 2020-05-12 , DOI: 10.1007/s11056-020-09792-4
Francival Cardoso Felix , Danilo Flademir Alves de Oliveira , Cibele dos Santos Ferrari , Eduardo Luiz Voigt , Mauro Vasconcelos Pacheco

The quality of forest seeds may be maintained in the course of storage for their use in the restoration of degraded areas and the conservation of genetic resources. In this sense, physiological and biochemical markers were evaluated during the short-term storage of Pityrocarpa moniliformis seeds, a pioneer species native to the Caatinga. Seeds harvested from 25 mother trees were stored in a growth chamber (27 ± 4 °C) or in a refrigerator (6 ± 3 °C) for 360 days. Viability, vigour, reserve contents, metabolite contents, and hydrolase activities were assessed at the beginning of the experiment and every 60 days. Over time, a decrease in germination percentage, germination speed index, and seedling growth accompanied by an increase in membrane damage was verified in the seeds kept in a growth chamber. These alterations were not associated with the degradation of starch and storage proteins, although there was a decrease in the content of non-reducing sugars in the seeds kept in the growth chamber. Therefore, the loss of viability and vigour in P. moniliformis seeds stored for 360 days in a growth chamber cannot be attributed to the hydrolysis of major reserves, but it may be related to the accumulation of reducing sugars.



中文翻译:

苦果李子种子短期储存过程中活力和活力的丧失不能归因于储备退化

在储存过程中可以保持森林种子的质量,以用于退化地区的恢复和遗传资源的保存。从这个意义上说,在苦瓜果肉的短期储存过程中对生理和生化指标进行了评估。种子,Caatinga原生的先锋物种。从25棵母树上收获的种子在生长室(27±4°C)或冰箱(6±3°C)中存储360天。在实验开始时和每60天评估一次活力,活力,储备含量,代谢产物含量和水解酶活性。随着时间的流逝,在生长室中保存的种子中,发芽率,发芽速度指数和幼苗生长都伴随着膜损伤的增加而降低。这些变化与淀粉和贮藏蛋白的降解无关,尽管生长室中保留的种子中非还原糖的含量有所减少。因此,念珠假单胞菌丧失活力和活力 在生长室中储存360天的种子不能归因于主要储备的水解,但可能与还原糖的积累有关。

更新日期:2020-05-12
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