当前位置: X-MOL 学术Nanoscale Res. Lett. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Detection of microRNA-335-5p on an Interdigitated Electrode Surface for Determination of the Severity of Abdominal Aortic Aneurysms.
Nanoscale Research Letters ( IF 5.418 ) Pub Date : 2020-05-11 , DOI: 10.1186/s11671-020-03331-y
Bei Lu 1 , Leiting Liu 2 , Jingrui Wang 1 , Yuan Chen 3 , Zhijiang Li 4 , Subash C B Gopinath 5, 6 , Thangavel Lakshmipriya 6 , Zongwei Huo 7
Affiliation  

Abdominal aortic aneurysm (AAA) refers to the enlargement of the lower artery of the abdominal aorta, and identification of an early detection tool is urgently needed for diagnosis. In the current study, an interdigitated electrode (IDE) sensing surface was used to identify miRNA-335-5p, which reflects the formation of AAAs. The uniformity of the silica material was observed by 3D profilometry, and the chemically modified highly conductive surface improved the detection via the I-V mode. The targeted miRNA-335-5p was detected in a dose-dependent manner and based on linear regression and 3σ analyses, the sensitivity was determined to be 1 fM with a biotinylated probe. The high specificity was shown by discriminating the target sequence from noncomplementary and single- and triple-mismatched sequences. These outputs demonstrated the high-performance detection of miRNA-335-5p with good reproducibility for determination of the severity of AAA.

中文翻译:

在指状电极表面检测microRNA-335-5p,以确定腹主动脉瘤的严重程度。

腹主动脉瘤(AAA)是指腹主动脉下动脉增大,因此急需使用早期检测工具进行诊断。在当前的研究中,使用叉指电极(IDE)感应表面来识别miRNA-335-5p,这反映了AAA的形成。通过3D轮廓测定法观察二氧化硅材料的均匀性,并且化学改性的高导电表面改善了通过IV模式的检测。以剂量依赖的方式检测靶向的miRNA-335-5p,并基于线性回归和3σ分析,使用生物素化探针确定的敏感性为1 fM。通过将靶序列与非互补序列和单错配和三重错配序列区分开来显示了高特异性。
更新日期:2020-05-11
down
wechat
bug