Rubus ellipticus Smith. commonly known as ‘Yellow Himalayan raspberry’ is an important member of Rosaceae family with high medicinal importance having antioxidant and antibacterial properties. This study was done with the objective of characterizing 21 R. ellipticus collections from different regions of India, based on morphological descriptors and expressed sequence tags-simple sequence repeat (EST-SSR) markers. Broad phenotypic variability among the R. ellipticus collections was detected by using morphological descriptors. The Euclidian distance matrix discriminated two groups, based on morphological characters. A set of 68 EST-SSR markers were developed based on 7777 unigenes generated by us from ‘Kumarhatti-1’ collection of R. ellipticus using assembled pair—end sequences from Illumina NextSeq 500. The ESTs containing simple sequence repeat motifs were extracted using Microsatellite identification tool and primers were designed. Out of 68 EST-SSRs polymorphism was detected using 61 (89.7%) primer pairs, indicating high genetic variability in the R. ellipticus collections. Effective multiplex ratio, marker index and polymorphism information content were 0.43, 0.24 and 0.50, respectively. The unweighted pair group method with arithmetic mean coincided with the bootstrap analysis and two groups were formed, separating the collections Uttrakhand from the other. Morphological descriptors along with EST-SSR markers proved efficient in detecting the levels of genetic variability among the collections maintained in the field. These results can be used as an additional source of exploitation in R. ellipticus breeding programs.

悬钩子属史密斯。俗称“黄色喜马拉雅山莓”是蔷薇科家族的重要成员，具有重要的药用价值，具有抗氧化和抗菌性能。进行这项研究的目的是基于形态学描述符和表达的序列标签-简单序列重复（EST-SSR）标记，表征来自印度不同地区的21个R. ellipticus集合。通过使用形态学描述符检测到椭圆形R.集合之间广泛的表型变异。根据形态特征，欧几里得距离矩阵将两组区分。基于我们从椭圆毛。的“ Kumarhatti-1”收集物中产生的7777个单基因，开发了一组68个EST-SSR标记使用来自Illumina NextSeq 500的组装的末端对序列。使用微卫星识别工具提取包含简单序列重复基序的EST，并设计引物。在68个EST-SSRs多态性中，使用61对（89.7％）引物对进行了检测，这表明椭圆轮虫具有很高的遗传变异性。集合。有效复用比，标记指数和多态信息含量分别为0.43、0.24和0.50。具有算术平均值的非加权对分组方法与自举分析相吻合，并形成了两组，将集合Uttrakhand彼此分开。形态描述子与EST-SSR标记一起被证明可以有效地检测田间保存的收集物中的遗传变异水平。这些结果可以用作椭圆红螺菌育种计划中的其他开发资源。