The numbers/densities of neurons and gliocytes and the neuron-to-glia ratios were calculated in different layers of the cerebral cortex of three subjects in a post mortem study. The subjects were 60 to 70 years old (which was qualified as a relatively early period of aging) and had no history of neurodegeneration diseases. The brains embalmed in 10% formalin-fixative solution were processed, and coronal 4-μm-thick slices of tissues of the frontal, parietal, temporal, and occipital lobes were prepared and stained with hematoxylin-eosin. Clear cortical lamination was observed in all lobes. In the frontal lobe, the mean neuron density (number of neurons within visual fields at a 400× magnification) was significantly higher, while that of gliocytes was significantly lower compared to the respective values in other lobes (P < 0.05). A significant association between the number of neurons and glia was found in the frontal lobe (r2 = 0.40, P = 0.0029) and in all superficial cortical layers (r2 = 0.72, P = 0.0025), but not in the deeper layers (r2 = 0.18, P = 0.14). The mean neuron-to-glia ratio in different lobes and layers varied from 1.0 : 0.81 to 1.0 : 3.54. It is concluded that, in early-aging subjects with no cerebral pathology, the quantitative characteristics of cortical neurons and glial cells do not differ significantly from those in the brains of younger adult healthy subjects.

中文翻译：

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人类衰老大脑皮层的细胞成分

在验尸研究中计算了三个受试者大脑皮层不同层的神经元和神经胶质细胞的数量/密度以及神经元与神经胶质的比率。受试者年龄在60～70岁（属于较早衰老期），无神经退行性疾病史。处理在 10% 福尔马林固定液中防腐的大脑，制备 4 μm 厚的额叶、顶叶、颞叶和枕叶组织的冠状切片，并用苏木精-伊红染色。在所有叶中观察到清晰的皮质分层。在额叶中，平均神经元密度（400 倍放大倍数下视野内的神经元数量）显着较高，而胶质细胞的平均神经元密度与其他叶中的相应值相比显着较低（P < 0.05）。在额叶 (r2 = 0.40, P = 0.0029) 和所有表层皮层 (r2 = 0.72, P = 0.0025) 中发现神经元和神经胶质数量之间存在显着关联，但在更深层 (r2 = 0.18，P = 0.14）。不同叶和层中神经元与神经胶质的平均比率从 1.0:0.81 到 1.0:3.54 不等。结论是，在没有脑部病变的早衰受试者中，皮质神经元和神经胶质细胞的数量特征与年轻健康受试者大脑中的数量特征没有显着差异。0.81 到 1.0：3.54。结论是，在没有脑部病变的早衰受试者中，皮质神经元和神经胶质细胞的数量特征与年轻健康受试者大脑中的数量特征没有显着差异。0.81 到 1.0：3.54。结论是，在没有脑部病变的早衰受试者中，皮质神经元和神经胶质细胞的数量特征与年轻健康受试者大脑中的数量特征没有显着差异。