Abstract—

The sequenced genome data from the sea urchin Strongylocentrotus purpuratus were used to identify transposons (TEs, transposable elements) and test for the presence of some of them in the genome of the sea urchin S. intermedius. The known TEs of S. purpuratus from the RepBase repeats database were used to calculate the primers and identify the corresponding TEs in the S. intermedius transcriptome. High-coverage TE sequences from the S. intermedius transcriptome were assembled based on comparison with the annotated TEs of the S. purpuratus genome. TEs with an insufficient number of reading frames in the transcriptome were beyond the analysis. The approach applied in the present study was successful: out of the 100 assembled TEs of S. intermedius, 92 corresponding TEs in the S. intermedius genome were identified using the calculated primer pairs. The TE sequences calculated by in silico methods are present in the S. intermedius genome and can be useful in further research.

中文翻译：

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海胆Strongylocentrotus intermedius Agassiz的转座子，1863年：计算机与体外

摘要—

来自海胆Strongylocentrotus purpuratus的测序基因组数据用于鉴定转座子（TEs，可转座元件）并测试其中一些在中间海胆链球菌中的存在。已知的转座因子S.紫石从RepBase重复序列数据库被用来计算所述的引物和识别相应的TE在S.中间型转录。基于与紫癜链霉菌注释的TE的比较，从中间链霉菌转录组提取高覆盖度的TE序列。基因组。转录组中阅读框数量不足的TE超出了分析范围。在本研究中应用的方法是成功的：在中间链球菌的100个组装TE中，使用计算出的引物对鉴定了中间链球菌基因组中的92个相应TE 。通过计算机方法计算的TE序列存在于中间链球菌基因组中，可用于进一步研究。