The present investigation was undertaken to assess the hybridity of experimental F₁ plants derived from the crossing of salinity and/or submergence tolerant landraces with the high-yielding popular varieties of the west coast region of India. Seven microsatellite markers were utilized for confirming the hybridity of the 16 F₁ plants developed from four cross combinations, viz. Karjat-3 X KS-19-2 (Cross 1), Goa dhan-3 X Jaddubatta (Cross 2), Jaya X KS-19-2 (Cross 3) and Karjat-3 X Jaddubatta (Cross 4). Polymorphism survey using above seven markers between the parents of all the four crosses revealed that six of them were polymorphic, whereas one marker was monomorphic. The marker RM21539 was found to be polymorphic in all the four cross combinations, followed by RM493, RM3412, RM206 and RM10843 exhibiting polymorphism in three crosses, while marker RM25181 was polymorphic in two crosses. The screening of F₁s with polymorphic markers revealed that the F₁s from Cross 3 exhibited heterozygosity (showing alleles from both the parents) at six marker loci, whereas the F₁s from Cross 1 and Cross 4 at five loci and Cross 2 at two marker loci, thereby confirming hybridity in all the 16 tested F₁ plants.

中文翻译：

---

利用微卫星标记评价水稻实验F 1的杂种

进行本研究以评估来自盐分和/或耐淹水地方品种与印度西海岸地区高产受欢迎品种的杂交实验F ₁植物的杂交性。七个微卫星标记用于确认16 F ₁的杂交从四个交叉组合开发的植物，即。Karjat-3 X KS-19-2（交叉1），果阿丹3 X Jaddubatta（交叉2），Jaya X KS-19-2（交叉3）和Karjat-3 X Jaddubatta（交叉4）。使用四个十字的亲本之间的以上七个标记进行的多态性调查显示，其中六个是多态的，而一个标记是单态的。发现标记RM21539在所有四个杂交组合中都是多态性的，其次是RM493，RM3412，RM206和RM10843在三个杂交中均表现出多态性，而标记RM25181在两个杂交中均具有多态性。用多态性标记物筛选F ₁ s显示，来自Cross 3的F ₁ s在六个标记位点处表现出杂合性（显示来自两个亲本的等位基因），而F ₁在五个位点上来自杂交1和杂交4，在两个标记位上来自杂交2，从而证实了在所有16个测试的F ₁植物中的杂交。