The epidemic of cholera in Siberia and the Russian Far East during the seventh pandemic was recorded as sporadic cases of introduction of the infection into the territory or severe local outbreaks associated with the infection’s importation in the 1970s and 1990s. The genetic diversity and relationship of 83 V. cholerae O1 El Tor strains isolated from the region with epidemic complications were studied on the basis of the multilocus analysis of the variable number tandem repeats at five loci: VcA, VcB, VcC, VcD, and VcG. The VcB locus was characterized by the maximum polymorphism in the sample of the studied strains, while the VcC locus displayed the minimum polymorphism. Cluster analysis revealed the differentiation of V. cholerae strains during epidemic complications into seven clusters depending on the structure of the pathogenic determinants (typical and atypical El Tor vibrios), as well as on the time and direction of the pathogen importation. In some outbreaks in the Far East in 1999, the heterogeneity of the etiological agent populations was shown (five genotypes were detected among the outbreak strains from Yuzhno-Sakhalinsk, and six genotypes were determined among the strains from Primorskii krai). At the same time, the predominant MLVA profiles and their single locus variants were identified. The absence of a strict connection between the territory and the genotypes of V. cholerae outbreak strains (one strain from Yuzhno-Sakhalinsk showed the identity of the allele profile with the isolates from Vladivostok), as well as no correspondence of the V. cholerae genotype to a specific infection focus, was shown. According to the data of the MLVA typing, the V. cholerae O1 El Tor strains isolated from the Siberian and Far Eastern regions (Russia) with epidemic complications were characterized by the heterogeneity of their genetic organization, which agrees with the period and direction of their importation to the territory. During sporadic outbreaks of cholera, the clonality of the etiological agent was revealed. Closely related subclones, the emergence of which could result from the presence of the causative agent in the environment or passaging through a susceptible organism, were identified.

中文翻译：

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西伯利亚和远东地区流行并发症期间霍乱弧菌的遗传多样性 О1 El Tor

第七次大流行期间西伯利亚和俄罗斯远东地区的霍乱流行被记录为在 1970 年代和 1990 年代将感染传入该领土的零星病例或与感染输入相关的严重局部爆发。在对 VcA、VcB、VcC、VcD 和 VcG 五个位点的可变数量串联重复序列进行多位点分析的基础上，研究了从流行并发症地区分离的 83 株霍乱弧菌 O1 El Tor 菌株的遗传多样性和相关性。 . VcB 基因座的特征在于所研究菌株样品中的最大多态性，而 VcC 基因座显示出最小的多态性。聚类分析揭示了 V 的分化。根据致病决定因素（典型和非典型 El Tor 弧菌）的结构以及病原体输入的时间和方向，将流行并发症期间的霍乱菌株分为七个集群。在1999年远东地区的一些暴发中，病原体种群的异质性表现出（在南萨哈林斯克暴发株中检测到5种基因型，在滨海边疆区的毒株中检测到6种基因型）。同时，确定了主要的 MLVA 谱及其单基因座变异。领土与霍乱弧菌爆发菌株的基因型之间缺乏严格的联系（来自南萨哈林斯克的一种菌株显示出与符拉迪沃斯托克分离株的等位基因图谱相同），并且没有对应的霍乱弧菌爆发菌株。显示了特定感染焦点的霍乱基因型。根据MLVA分型数据，从西伯利亚和远东地区（俄罗斯）分离的具有流行并发症的霍乱弧菌O1 El Tor菌株具有遗传组织异质性的特点，这与它们的时期和方向一致。进口到领土。在霍乱的零星爆发期间，病原体的克隆性被揭示出来。确定了密切相关的亚克隆，它们的出现可能是由于环境中存在病原体或通过易感生物传代所致。从西伯利亚和远东地区（俄罗斯）分离出的具有流行并发症的霍乱 O1 El Tor 菌株的特点是其遗传组织的异质性，这与它们输入该领土的时期和方向一致。在霍乱的零星爆发期间，病原体的克隆性被揭示出来。确定了密切相关的亚克隆，它们的出现可能是由于环境中存在病原体或通过易感生物传代所致。从西伯利亚和远东地区（俄罗斯）分离出的具有流行并发症的霍乱 O1 El Tor 菌株的特点是其遗传组织的异质性，这与它们输入该领土的时期和方向一致。在霍乱的零星爆发期间，病原体的克隆性被揭示出来。确定了密切相关的亚克隆，它们的出现可能是由于环境中存在病原体或通过易感生物传代所致。